ArciTect™ T7 Endonuclease I Kit
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ArciTect™ T7 Endonuclease I Kit -
ArciTect™ T7 Endonuclease I Kit -
Label for ArciTect™ T7 Endonuclease I Kit -
Label for ArciTect™ T7 Endonuclease I Kit -
Label for ArciTect™ T7 Endonuclease I Kit
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Required Products
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DNase- and RNase-free water for molecular biology applications -
ArciTect™ High-Fidelity DNA Polymerase Kit
Polymerase, buffers, and dNTPs for high-fidelity PCR amplification -
For removing nucleases from DNA/RNA samples and terminating enzymatic reactions -
For size determination and tracking of double-stranded DNA products by gel electrophoresis
Overview
Related Products
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Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing -
Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing -
Custom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing -
ArciTect™ Human HPRT Positive Control Kit
Positive control for CRISPR-Cas9 genome editing
Scientific Resources
Product Documentation
Educational Materials
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. INDEL Detection by T7 Endonuclease I Assay
Human embryonic stem (ES) and induced pluripotent stem (iPS) cells (A) and T cells (B) were edited using ArciTect™ Cas9 Nuclease (Catalog #76002) and ArciTect™ Human HPRT Positive Control Kit (Catalog #76013), and INDEL formation was assessed using ArciTect™ T7 Endonuclease I Kit. Following CRISPR-mediated editing at the HPRT locus, genomic DNA was isolated and a 1 kb region surrounding the target site was amplified by PCR using ArciTect™ Human HPRT Primer Mix (included with Catalog #76013). PCR products were purified, then denatured, re annealed, and cut with ArciTect™ T7 Endonuclease I. Samples were resolved on a 1% agarose gel, and band intensities were determined using a ChemiDoc™ MP Imaging System (Bio-Rad). Relative intensities of the full length (1083 base pairs [bp]) and T7 cleavage product bands (827 and 256 bp) were used to calculate the cleavage efficiency (%). Control: Uncut PCR product (no T7 added); Test, Donor 1, and Donor 2: T7-digested PCR product.