ArciTect™ Human HPRT Positive Control Kit

Positive control for CRISPR-Cas9 genome editing

ArciTect™ Human HPRT Positive Control Kit

Positive control for CRISPR-Cas9 genome editing

ArciTect™ Human HPRT Positive Control Kit
1 Kit
303 USD
Catalog # 76013

Positive control for CRISPR-Cas9 genome editing

What's Included

  • ArciTect™ Human HPRT crRNA, 2 nmol
  • ArciTect™ Human HPRT Primer Mix, 2 nmol
  • ArciTect™ tracrRNA, 5 nmol
  • ArciTect™ Annealing Buffer (5X), 100 µL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

ArciTect™ Human HPRT Positive Control Kit is designed as a positive control for experiments using the ArciTect™ CRISPR-Cas9 genome editing system. The kit comprises ArciTect™ Human HPRT crRNA (2 nmol), ArciTect™ Human HPRT Primer Mix (2 nmol), ArciTect™ tracrRNA (5 nmol), and ArciTect™ Annealing Buffer (5X). ArciTect™ Human HPRT crRNA first requires annealing to ArciTect™ tracrRNA (included in the kit). This must then be combined with an ArciTect™ Cas9 Nuclease (e.g. Catalog #76002) to form a ribonucleoprotein complex. ArciTect™ Human HPRT Primer Mix can be used to amplify genomic DNA isolated from a population of transfected cells, which can subsequently be used in a T7 endonuclease I assay to determine cleavage efficiency.
This kit has been tested and validated for use with the ArciTect™ line of genome editing products. HPRT, or hypoxanthine phosphoribosyltransferase, is a housekeeping gene and a commonly used control. The kit can be used to optimize transfection protocols and act as a positive control that can be used alongside custom ArciTect™ crRNAs (e.g. Catalog #76010).
Cell Type
Pluripotent Stem Cells
Species
Human
Brand
ArciTect
Area of Interest
Stem Cell Biology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
76013
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
76013
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
76013
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
76013
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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