Dispase, Animal Component-Free (ACF) is a neutral, amino-endoprotease that cleaves the N-terminal peptide bond of non-polar amino acid residues and is obtained from cultures free of animal-derived materials. Dispase has mild proteolytic activity, which makes it especially useful for the isolation and passaging of primary cells. Its proteolytic activity also allows it to maintain membrane integrity. Dispase is commonly used with other proteases such as collagenase in cell isolation and tissue dissociation for neural tissue (Dietrich et al.), kidney (Presnell et al.), epithelial tissue (Smoot et al.), endothelial tissue (Müller et al.), lung (Barkauskas et al.), colon (Roig et al.), and stem cells (Thomson et al.; Salmon et al.).