Mammary Epithelial Cell Research

Mammary stem cells along with mammary luminal and myoepithelial progenitor cells, are found in the mammary gland and undergo expansion and differentiation when required to support the organ’s morphological changes during development, puberty, pregnancy, lactation and involution. See MoreIsolate mammary stem and progenitor cells from mammary tissue with our enzymatic dissociation reagents to generate a single cell suspension. Enrich for the desired cell type using one of our column-free immunomagnetic cell isolation kits that are designed for mammary cell research. This produces a highly pure sample ready for cell culture and mammary colony-forming unit (Ma-CFU) assays

Learn More

Mini Review Mammary Stem Cells and Breast Cancer
Video (1:23) Tools for Breast Cancer Research
Technical Bulletin Monolayer Culture of Mouse Mammary Epithelial Cells
Technical Bulletin Identification of ALDH-Expressing Cancer Stem Cells using ALDEFLUOR™

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Products

Cell Culture Media and Supplements (4 6)

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EpiCult™-B Mouse Medium Kit

For culture and evaluation of mouse mammary epithelial cells
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EpiCult™-B Human Medium Kit

For culture and evaluation of human mammary epithelial cells in CFU assays
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EpiCult™-C Human Medium Kit

For culture of human mammary epithelial cells
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DMEM/F-12 with 15 mM HEPES

Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

Cell Isolation Products (4 3)

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EasySep™ Human EpCAM Positive Selection Kit II

Immunomagnetic positive selection kit
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EasySep™ Mouse Epithelial Cell Enrichment Kit II

Immunomagnetic negative selection kit
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RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36

Immunodensity negative selection cocktail

Cell Dyes and Detection Assay Kits (4 3)

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ALDEFLUOR™ Kit

For the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH
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Carmine Alum

Dye for staining mouse mammary gland whole mounts
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DAPI (Hydrochloride)

DNA-labeling dye

Tissue and Cell Culture Dissociation Reagents (4 20)

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DNase I Solution (1 mg/mL)

Cell dissociation reagent
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Dispase, ACF

Animal component-free non-specific protease
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Dispase (1 U/mL)

1 U/mL dispase in DMEM/F-12
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Gentle Collagenase/Hyaluronidase

10X Gentle collagenase/hyaluronidase in DMEM

Buffers and Solutions (4 4)

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Ammonium Chloride Solution

Reagent for lysis of red blood cells
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HBSS with 10 mM HEPES, Without Phenol Red

Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red
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EasySep™ Buffer

Cell separation buffer
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RoboSep™ Buffer

Cell separation buffer

Cytokines and Proteins (4 12)

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Human Recombinant FGF-7, ACF

Fibroblast growth factor 7, animal component-free
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Human Recombinant FGF-10 (KGF-2), ACF

Fibroblast growth factor 10, animal component-free
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Human Recombinant EGFR

Epidermal growth factor receptor
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Human Recombinant EGF, ACF

Epidermal growth factor, animal component-free

Antibodies (4 1)

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Anti-Human CD73 (Ecto-5'-Nucleotidase) Antibody, Clone AD2

Mouse monoclonal IgG1 antibody against human, rhesus, chimpanzee CD73

Cultureware and General Supplies (4 2)

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Tissue Dissociation Flask

Borosilicate glass flat-bottom flask for enzymatic digestion of tissue
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Cell Culture Flask

Tissue culture-treated cell culture flask; T-75, T-150 and T-175 cm² formats

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