Mammary Epithelial Cell Research
Mammary stem cells along with mammary luminal and myoepithelial progenitor cells, are found in the mammary gland and undergo expansion and differentiation when required to support the organ’s morphological changes during development, puberty, pregnancy, lactation and involution.
Products
Cell Culture Media and Supplements (4 6)
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EpiCult™-B Mouse Medium Kit
For culture and evaluation of mouse mammary epithelial cells -
EpiCult™-B Human Medium Kit
For culture and evaluation of human mammary epithelial cells in CFU assays -
EpiCult™-C Human Medium Kit
For culture of human mammary epithelial cells -
DMEM/F-12 with 15 mM HEPES
Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
Cell Isolation Products (4 3)
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EasySep™ Human EpCAM Positive Selection Kit II
Immunomagnetic positive selection kit -
EasySep™ Mouse Epithelial Cell Enrichment Kit II
Immunomagnetic negative selection kit -
RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36
Immunodensity negative selection cocktail
Cell Dyes and Detection Assay Kits (4 3)
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ALDEFLUOR™ Kit
For the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH -
Carmine Alum
Dye for staining mouse mammary gland whole mounts -
DAPI (Hydrochloride)
DNA-labeling dye
Tissue and Cell Culture Dissociation Reagents (4 20)
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DNase I Solution (1 mg/mL)
Cell dissociation reagent -
Dispase, ACF
Animal component-free non-specific protease -
Dispase (1 U/mL)
1 U/mL dispase in DMEM/F-12 -
Gentle Collagenase/Hyaluronidase
10X Gentle collagenase/hyaluronidase in DMEM
Buffers and Solutions (4 5)
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Ammonium Chloride Solution
Reagent for lysis of red blood cells -
ALDEFLUOR™ Assay Buffer
Assay buffer for labeling with the ALDEFLUOR™ kit -
HBSS with 10 mM HEPES, Without Phenol Red
Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red -
EasySep™ Buffer
Cell separation buffer
Cytokines and Proteins (4 12)
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Human Recombinant FGF-7, ACF
Fibroblast growth factor 7, animal component-free -
Human Recombinant FGF-10 (KGF-2), ACF
Fibroblast growth factor 10, animal component-free -
Human Recombinant EGFR
Epidermal growth factor receptor -
Human Recombinant EGF, ACF
Epidermal growth factor, animal component-free
Antibodies (4 1)
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Anti-Human CD73 (Ecto-5'-Nucleotidase) Antibody, Clone AD2
Mouse monoclonal IgG1 antibody against human, rhesus, chimpanzee CD73
Cultureware and General Supplies (4 2)
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Tissue Dissociation Flask
Borosilicate glass flat-bottom flask for enzymatic digestion of tissue -
Cell Culture Flask
Tissue culture-treated cell culture flask; T-75, T-150 and T-175 cm² formats