Mammary Epithelial Cell Research

Mammary stem cells along with mammary luminal and myoepithelial progenitor cells, are found in the mammary gland and undergo expansion and differentiation when required to support the organ’s morphological changes during development, puberty, pregnancy, lactation and involution. See MoreIsolate mammary stem and progenitor cells from mammary tissue with our enzymatic dissociation reagents to generate a single cell suspension. Enrich for the desired cell type using one of our column-free immunomagnetic cell isolation kits that are designed for mammary cell research. This produces a highly pure sample ready for cell culture and mammary colony-forming unit (Ma-CFU) assays

Learn More

Mini Review Mammary Stem Cells and Breast Cancer
Video (1:23) Tools for Breast Cancer Research
Technical Bulletin Monolayer Culture of Mouse Mammary Epithelial Cells
Technical Bulletin Identification of ALDH-Expressing Cancer Stem Cells using ALDEFLUOR™

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Products

Cell Culture Media and Supplements (42)

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EpiCult™-B Mouse Medium Kit

For culture and evaluation of mouse mammary epithelial cells
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DMEM/F-12 with 15 mM HEPES

Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

Cell Isolation Products (41)

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EasySep™ Mouse Epithelial Cell Enrichment Kit II

Immunomagnetic negative selection kit

Cell Dyes and Detection Assay Kits (43)

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ALDEFLUOR™ Kit

For the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH
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Carmine Alum

Dye for staining mouse mammary gland whole mounts
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DAPI (Hydrochloride)

DNA-labeling dye

Tissue and Cell Culture Dissociation Reagents (417)

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Dispase, ACF

Animal component-free non-specific protease
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Dispase (1 U/mL)

1 U/mL dispase in DMEM/F-12
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DNase I, ACF

Animal component-free DNase I for digestion of DNA
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DNase I

For digestion of DNA

Buffers and Solutions (45)

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Ammonium Chloride Solution

Reagent for lysis of red blood cells
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ALDEFLUOR™ Assay Buffer

Assay buffer for labeling with the ALDEFLUOR™ kit
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HBSS with 10 mM HEPES, Without Phenol Red

Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red
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EasySep™ Buffer

Cell separation buffer

Cytokines and Proteins (41)

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Mouse Recombinant EGF

Epidermal growth factor

Cultureware and General Supplies (42)

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Tissue Dissociation Flask

Borosilicate glass flat-bottom flask for enzymatic digestion of tissue
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Cell Culture Flask

Tissue culture-treated cell culture flask with vent cap; T-150 and T-175 cm² formats

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