Mammary Epithelial Cell Research

Mammary epithelial progenitor cells undergo differentiation to form luminal and myoepithelial cells, the two major epithelial cell types of the mammary gland. See More Expand isolated mammary progenitor cells as monolayer cultures, evaluate them in mammary colony-forming units (Ma-CFUs) or three-dimensional gel-based cultures, or generate mammosphere or tumorsphere cultures to provide a more physiologically relevant environment. For mammosphere and tumorsphere cultures, use the most published commercially available medium designed for this purpose and study cells isolated from normal and cancerous breast tissue.

Learn More

Technical Bulletin Monolayer Culture of Human Mammary Epithelial Cells
Technical Bulletin Tumorsphere culture of human breast cancer cell lines
Video (2:05) Counting 3D Sphere Cultures
Mini Review Mammary Stem Cells and Breast Cancer

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Products

Cell Culture Media and Supplements (4 8)

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EpiCult™ Plus Medium

For culture of human, mouse, and rat epithelial stem cells
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MammoCult™ Human Medium Kit

For culture of human mammospheres and tumorspheres
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EpiCult™-C Human Medium Kit

For culture of human mammary epithelial cells
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EpiCult™-B Human Medium Kit

For culture and evaluation of human mammary epithelial cells in CFU assays

Buffers and Solutions (4 1)

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HBSS with 10 mM HEPES, Without Phenol Red

Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red

Cytokines and Proteins (4 18)

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Human Recombinant FGF-7, ACF

Fibroblast growth factor 7, animal component-free
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Human Recombinant FGF-10 (KGF-2), ACF

Fibroblast growth factor 10, animal component-free
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Human Recombinant EGFR

Epidermal growth factor receptor
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Human Recombinant EGF, ACF

Epidermal growth factor, animal component-free

Small Molecules (4 10)

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Tamoxifen

Estrogen signaling pathway modulator; Modulates selective estrogen receptor and estrogen-related receptor
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NU7026

NHEJ pathway inhibitor; Inhibits DNA-dependent protein kinase (DNA-PK)
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BSI-201

Cysteine-containing protein modifier; Inhibits growth of triple-negative breast cancer cells
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NU7441

NHEJ pathway inhibitor; Inhibits DNA-dependent protein kinase (DNA-PK)

Matrices and Substrates (4 1)

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Collagen Solution

For preparation of collagen gels and for coating cell culture surfaces

Tissue and Cell Culture Dissociation Reagents (4 2)

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Dispase (1 U/mL)

1 U/mL dispase in DMEM/F-12
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Trypsin-EDTA (0.25%)

Enzymatic cell dissociation reagent

Cell Dyes and Detection Assay Kits (4 2)

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Trypan Blue

Reagent for counting viable mammalian cells
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DAPI (Hydrochloride)

DNA-labeling dye

Cultureware and General Supplies (4 4)

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Cell Culture Flask

Tissue culture-treated cell culture flask; T-75, T-150 and T-175 cm² formats
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Ultra-Low Adherent Plate for Suspension Culture

Sterile, flat-bottom plate, with ultra-low adherent surface for suspension cultures, with lid; 6-well format
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AggreWell™400

Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids
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AggreWell™800

Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids

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