EasySep™ Mouse Epithelial Cell Enrichment Kit II
Immunomagnetic negative selection kit
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EasySep™ Mouse Epithelial Cell Enrichment Kit II -
Label for EasySep™ Mouse Epithelial Cell Enrichment Kit II
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Required Products
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HBSS with 10 mM HEPES, Without Phenol Red
Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red -
Magnet for column-free immunomagnetic separation -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Mouse Epithelial Cell Enrichment Kit II is designed to isolate epithelial cells from freshly dissociated mouse mammary tissues by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted non-epithelial cells with biotinylated antibodies and magnetic particles. The magnetically labeled cells are separated from untouched desired cells by using an EasySep™ magnet and simply pouring the desired cells into a new tube.
This product replaces the EasySep™ Epithelial Cell Enrichment Kit (Catalog #19758) for even faster isolations. It also replaces the cell enrichment portion of the EasySep™ Mouse Mammary Stem Cell Enrichment Kit (Catalog #19757). Additionally, Anti-Mouse CD24 Antibody, Clone M1/69, PE (Catalog #60099PE) and Anti-Mouse CD49f Antibody, Clone GoH3, FITC (Catalog #60037FI) are required to complete the kit.
This product replaces the EasySep™ Epithelial Cell Enrichment Kit (Catalog #19758) for even faster isolations. It also replaces the cell enrichment portion of the EasySep™ Mouse Mammary Stem Cell Enrichment Kit (Catalog #19757). Additionally, Anti-Mouse CD24 Antibody, Clone M1/69, PE (Catalog #60099PE) and Anti-Mouse CD49f Antibody, Clone GoH3, FITC (Catalog #60037FI) are required to complete the kit.
Advantages:
• Fast, easy-to-use and column-free
• Isolated cells are untouched
• Isolated cells are untouched
Components:
- EasySep™ Mouse Epithelial Cell Enrichment Kit II (Catalog #19868)
- EasySep™ Mouse Epithelial Cell Enrichment Cocktail, 0.5 mL
- EasySep™ Biotin Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype:
Cell Isolation Kits
Cell Type:
Mammary Cells; Prostate Cells
Species:
Mouse
Sample Source:
Other; Primary
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Epithelial Cell Biology
Related Products
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For culture and evaluation of mouse mammary epithelial cells -
Gentle Collagenase/Hyaluronidase
10X Gentle collagenase/hyaluronidase in DMEM
Labeling Antibodies
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Anti-Mouse CD49f Antibody, Clone GoH3
Rat monoclonal IgG2a antibody against human, mouse, rhesus CD49f (integrin α6) -
Anti-Mouse CD24 Antibody, Clone M1/69
Rat monoclonal IgG2b antibody against mouse CD24
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
Educational Materials
(4)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Starting with mouse mammary tissues, the epithelial cell content of the enriched fraction is typically 96.97 ± 0.54% (mean ± SD using the purple EasySep™ Magnet). In the above example, the percentages of epithelial cells in the start and final enriched fractions are 19.9% and 97.4%, respectively.
Publications
(1)
Nature communications 2019
Sterol regulatory element binding protein 1 couples mechanical cues and lipid metabolism.
Abstract
Abstract
Sterol regulatory element binding proteins (SREBPs) are a family of transcription factors that regulate lipid biosynthesis and adipogenesis by controlling the expression of several enzymes required for cholesterol, fatty acid, triacylglycerol and phospholipid synthesis. In vertebrates, SREBP activation is mainly controlled by a complex and well-characterized feedback mechanism mediated by cholesterol, a crucial bio-product of the SREBP-activated mevalonate pathway. In this work, we identified acto-myosin contractility and mechanical forces imposed by the extracellular matrix (ECM) as SREBP1 regulators. SREBP1 control by mechanical cues depends on geranylgeranyl pyrophosphate, another key bio-product of the mevalonate pathway, and impacts on stem cell fate in mouse and on fat storage in Drosophila. Mechanistically, we show that activation of AMP-activated protein kinase (AMPK) by ECM stiffening and geranylgeranylated RhoA-dependent acto-myosin contraction inhibits SREBP1 activation. Our results unveil an unpredicted and evolutionary conserved role of SREBP1 in rewiring cell metabolism in response to mechanical cues.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.