mTeSR™ Plus

cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells

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298 USD


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cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells
298 USD

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mTeSR™ Plus is a serum-free, stabilized cell culture medium for the feeder-free maintenance and expansion of human embryonic stem (ES) and induced pluripotent stem (iPS) cells. It is based on the mTeSR™1 formulation, the most widely published feeder-free cell culture medium for human ES and iPS cells.

To enhance cell-quality attributes during restricted feeds, critical medium components have been stabilized, including FGF2, and medium pH is more consistent. As a result, mTeSR™ Plus allows for greater cell expansion rates with daily feeding, while also maintaining cell quality during restricted feeding schedules.

mTeSR™ Plus is compatible with a variety of culture matrices, including Corning® Matrigel® hESC-Qualified Matrix (Corning Catalog #354277) and Vitronectin XF™ (Catalog #07180, a matrix developed and manufactured by Nucleus Biologics).

Each lot of mTeSR™ Plus 5X Supplement is used to prepare complete mTeSR™ Plus medium and then performance-tested in a culture assay using human pluripotent stem cells (hPSCs).

mTeSR™ Plus (Catalog #100-0276) is manufactured under relevant cGMPs, ensuring the highest quality and consistency for reproducible results. For additional quality information, visit
⦁ Enhanced buffering and stabilized FGF2 support cell quality while allowing for alternate feeding schedules
⦁ Supports superior culture morphology and cell growth characteristics
⦁ Enables heightened single-cell survival when used with CloneR™
⦁ Fully compatible with established genome editing and differentiation protocols
  • mTeSR™ Plus Kit (Catalog #05825)
    • mTeSR™ Plus Basal Medium, 400 mL
    • mTeSR™ Plus 5X Supplement, 100 mL
  • mTeSR™ Plus Kit, cGMP (Catalog #100-0276)
    • mTeSR™ Plus Basal Medium, 400 mL
    • mTeSR™ Plus 5X Supplement, 100 mL
Specialized Media
Cell Type:
Pluripotent Stem Cells
Cell Culture; Expansion; Maintenance
Area of Interest:
Disease Modeling; Drug Discovery and Toxicity Testing; Stem Cell Biology

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Data and Publications


Figure 1. mTeSR™ Plus Maintains Optimal pH Levels Throughout a Weekend-Free Protocol

The pH of spent medium from hPSCs cultured in mTeSR™ Plus is higher than that of hPSCs cultured in mTeSR™1 and other flexible-feeding medium at similar cell densities. pH and cell numbers were measured after a 72-hour period without feeding. Range of cell numbers shown represent different densities that would be observed throughout a typical passage. This demonstrates that feeds can be skipped for two days at any time during routine maintenance using mTeSR™ Plus while maintaining a pH above 7.0. Note: Cultures were fed double the standard medium volume prior to the 72-hour period without feeds in all media and cell numbers are from one well of a 6-well plate.

Figure 2. mTeSR™ Plus Maintains Consistent Levels of FGF2 Throughout a Weekend-Free Protocol

FGF2 levels remain high in mTeSR™ Plus when cultured at 37°C over a 72 hour time period. Measured by ELISA.

Figure 3. mTeSR™ Plus Supports Higher Cell Numbers

Growth curves were obtained for human ES (H9) cells cultured in mTeSR™1 or mTeSR™ Plus on Corning® Matrigel® matrix over 7 days with either daily feeds or restricted feeds. Growth curves were determined by seeding 20,000 cells per well of a 6-well plate as aggregates and counting the cell numbers each day in duplicate wells.

Figure 4. Larger Colonies are Observed in mTeSR™ Plus Cultures

The average colony size per passage (± SEM) was obtained for human ES (H1, H9) and iPS (STiPS-M001, WLS-1C) cells cultured in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds) on Corning® Matrigel® over 10 passages. Size was determined by measuring representative colony diameters at harvest. Note that this data is representative of cultures passaged at a 7-day passaging interval; smaller colony size should be expected if using shorter passaging intervals.

Figure 5. Normal human ES and iPS Cell Morphology is Observed in mTeSR™ Plus Cultures

Images depict undifferentiated human hES (H1) and iPS (WLS-1C) cells cultured on Corning®️ Matrigel®️ matrix in mTeSR™1 with daily feeds or mTeSR™ Plus with restricted feeds. Cells retain the prominent nucleoli and high nuclear-to-cytoplasmic ratio characteristic of this cell type after 10 passages. Densely packed cells and multi-layering are prominent when cells are ready to be passaged.

Figure 6. Cells Cultured in mTeSR™ Plus Medium with Restricted Feeding Express Undifferentiated Cell Markers

Human ES (H1, H9) and iPS (WLS-1C, STiPS-M001) cells were characterized using flow cytometry for undifferentiated cell markers, (A) OCT3/4 and (B) TRA-1-60. Graphs show average expression (± SEM) results from analyses of duplicate wells every 5 passages for up to 10-15 passages in mTeSR™1 (daily feeds), or mTeSR™ Plus (restricted feeds).

Figure 7. Cells Maintained in mTeSR™ Plus with Restricted Feeding Have Comparable Differentiation Efficiencies to Cells Maintained in mTeSR™1

Human ES (H1, H9) and iPS (WLS-1C, STiPS-M001) cells were maintained in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds). Cells were differentiated using directed differentiation protocols and subjected to flow cytometry analysis. Graphs show average expression (± SEM) results from the 4 cell lines. The markers used for flow cytometry for each germ layer are listed in the bar titles.

Figure 8. hPSCs Cultured in mTeSR™ Plus with Restricted Feeding Maintain a Normal Karyotype

Karyograms of (A) human ES (H1) and (B) iPS (WLS-1C) cells cultured in mTeSR™ Plus for 30 passages shows a normal karyotype is retained.

Figure 9. High Cloning Efficiency of hPSCs in mTeSR™ Plus Supplemented with CloneR™

hPSCs (H1, H9, WLS-1C, and STiPS-M001) plated in mTeSR™ Plus with CloneR™ demonstrate cloning efficiencies equal to or greater than hPSCs in mTeSR™1 with CloneR™. Cells were seeded at clonal density (25 cells/cm²) in mTeSR™1 or mTeSR™ Plus on CellAdhere™ Vitronectin™ XF™-coated plates. n ≧ 3 biological replicates.