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mTeSR™1

cGMP, feeder-free maintenance medium for human ES and iPS cells

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

mTeSR™1

cGMP, feeder-free maintenance medium for human ES and iPS cells

From: 364 USD
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cGMP, feeder-free maintenance medium for human ES and iPS cells
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What's Included

  • mTeSR™1 Complete Kit (Catalog #85850)
    • mTeSR™1 Basal Medium, 400 mL
    • mTeSR™1 5X Supplement, 100 mL
  • mTeSR™1 Complete Kit, 1 L (Catalog #85857)
    • mTeSR™1 Basal Medium, 800 mL
    • mTeSR™1 5X Supplement, 100 mL, 2 Bottles

What Our Scientist Says

It makes me proud knowing that my work is critical to keeping thousands of hPSC lines reliably healthy and consistent around the world.

Arwen HunterAssociate Director, Stem Cell Biology
Arwen Hunter, Associate Director, Stem Cell Biology

Overview

Use this specialized, feeder-free culture medium to achieve more consistent human pluripotent stem cell (hPSC) cultures with homogenous, undifferentiated phenotypes.

Manufactured under relevant cGMPs, mTeSR™1 ensures the highest quality and consistency for reproducible results in your fundamental research, as well as for cell therapy and investigational new drug research applications. This serum-free, complete cell culture medium is made with pre-screened raw materials to ensure batch-to-batch consistency and robust performance in feeder-free hPSC culture.

Use established protocols for applications ranging from derivation to differentiation with this most widely published feeder-free hPSC culture medium, which has been used by leading pluripotent stem cell researchers to successfully maintain thousands of hPSC lines in over 50 countries. For enhanced cell performance and versatile maintenance, you may also be interested in mTeSR™ Plus medium, which is also manufactured under relevant cGMPs and features stabilized components and enhanced buffering.

To request a Letter of Authorization (LOA) for the FDA Master File for mTeSR™1, click here.
Subtype
Specialized Media
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Expansion, Maintenance
Brand
TeSR
Area of Interest
Stem Cell Biology
Formulation Category
Serum-Free

Data Figures

Figure 1. Normal hES and hiPS Cell Morphology is Observed in cGMP mTeSR™1 Cultures

Undifferentiated (A) H1 human embryonic stem (hES) and (B) WLS-1C human induced pluripotent stem (hiPS) cells cultured on Corning® Matrigel® Matrix in cGMP mTeSR™1 retain the prominent nucleoli and high nuclear-to-cytoplasmic ratio characteristic of this cell type after 10 passages. Densely packed cells and multi-layering are prominent when cells are ready to be passaged.

Figure 2. High Expansion Rates are Observed in cGMP mTeSR™1 Cultures

Graph shows the average fold expansion per passage +/- SEM obtained for hES (H1 and H9) and hiPS (WLS-1C) cells cultured in cGMP mTeSR­™1 (red) or non-cGMP mTeSR™1 (gray) on Corning® Matrigel® Matrix over 10 passages. Expansion was determined by enumerating the cell aggregates obtained at harvest and dividing by the number of cell aggregates seeded. Note that this data is representative of cultures passaged after 6-7 days in culture, lower expansion should be expected if using shorter culture times.

Figure 3. Cells Cultured in cGMP mTeSR™1 Medium Express Undifferentiated Cell Markers

Histogram analysis for hES (H1 and H9) and hiPS (WLS-1C) cells characterized using FACS for undifferentiated cell markers, OCT4 (OCT3) (Catalog #60093) and TRA-1-60 (Catalog #60064), after 8 - 10 passages in cGMP mTeSR™1 (filled = sample, blank = isotype control).

Figure 4. hPSCs Maintained in cGMP mTeSR™1 Display a Normal Karyotype

Karyograms of (A) H1 hES and (B) WLS-1C hiPS cells cultured in cGMP mTeSR™1 for 11 passages shows that a normal karyotype is retained.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
mTeSR™1
Catalog #
85857, 85850
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
mTeSR™1
Catalog #
85850
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
mTeSR™1
Catalog #
85857, 85850
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
mTeSR™1
Catalog #
85857, 85850
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
mTeSR™1
Catalog #
85857, 85850
Lot #
All
Language
English
The Certificate of Analysis for this product has been updated for newly released materials. To access respective CoAs please use this tool.

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (40)

Maximize Your Pluripotential with the TeSR™ Family of hPSC Culture Media
Brochure
Maximize Your Pluripotential with the TeSR™ Family of hPSC Culture Media
mTeSR™3D Suspension Culture Medium for hPSCs
Brochure
mTeSR™3D Suspension Culture Medium for hPSCs
Products for Human Pluripotent Stem Cells
Brochure
Products for Human Pluripotent Stem Cells
cGMP mTeSR™1
Brochure
cGMP mTeSR™1
qPCR Arrays for Cell Characterization
Brochure
qPCR Arrays for Cell Characterization
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Brochure
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSR™1 or TeSR™-E8™
Technical Bulletin
Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSR™1 or TeSR™-E8™
Stem Cell States: Naive to Primed Pluripotency
Wallchart
Stem Cell States: Naive to Primed Pluripotency
Derivation and Applications of Human Pluripotent Stem Cells
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Pluripotent Stem Cell Biology
Wallchart
Pluripotent Stem Cell Biology
STEMCELL Journal Club: Cerebral Organoids for Human-Specific Infection Modeling
28:45
Video
STEMCELL Journal Club: Cerebral Organoids for Human-Specific Infection Modeling
mTeSR™1: Standardized Medium for the Feeder-Independent Maintenance of hESCs & hiPSCs
1:27
Video
mTeSR™1: Standardized Medium for the Feeder-Independent Maintenance of hESCs & hiPSCs
How to Set Up an Assay with the hPSC Genetic Analysis Kit Experiment
8:33
Video
How to Set Up an Assay with the hPSC Genetic Analysis Kit Experiment
How to Count hPSC Aggregates to Determine Plating Density for Maintenance and Differentiation
3:39
Video
How to Count hPSC Aggregates to Determine Plating Density for Maintenance and Differentiation
How to Grow Cerebral Organoids from Human Pluripotent Stem Cells
9:18
Video
How to Grow Cerebral Organoids from Human Pluripotent Stem Cells
A Guide to Passaging Human Pluripotent Stem Cells Using mTeSR™1
5:58
Video
A Guide to Passaging Human Pluripotent Stem Cells Using mTeSR™1
Limitless Potential: Do More with TeSR™
3:10
Video
Limitless Potential: Do More with TeSR™
mTeSR™1: The Most Published, Feeder-Independent hESC & hiPSC Maintenance Medium
1:45
Video
mTeSR™1: The Most Published, Feeder-Independent hESC & hiPSC Maintenance Medium
Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration
21:35
Webinar
Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration
Building Brain Organoids and AssemBloids™ to Study Human Development and Disease
1:02:03
Webinar
Building Brain Organoids and AssemBloids™ to Study Human Development and Disease
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenance of Human Pluripotent Stem Cells (hPSCs)
42:10
Webinar
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenanc...
Nature Research Round Table: hPSC Lines for Cell Therapies - Panel Discussion
29:03
Webinar
Nature Research Round Table: hPSC Lines for Cell Therapies - Panel Discussion
Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells
19:35
Webinar
Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells
Nature Research Round Table: HLA Typing Considerations for Human Pluripotent Stem Cell Banking
13:01
Webinar
Nature Research Round Table: HLA Typing Considerations for Human Pluripotent Stem Cell Banking
Brains in a Dish: Using Cerebral Organoids to Study Human Brain Development and Disease
25:30
Webinar
Brains in a Dish: Using Cerebral Organoids to Study Human Brain Development and Disease
Quality Control Guidelines for Clinical-Grade Human Induced Pluripotent Stem Cell Lines
1:13:44
Webinar
Quality Control Guidelines for Clinical-Grade Human Induced Pluripotent Stem Cell Lines
Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types
1:07:14
Webinar
Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types
Genome Editing From Modeling Disease to Novel Therapeutics
42:37
Webinar
Genome Editing From Modeling Disease to Novel Therapeutics
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells
24:46
Webinar
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
13:02
Webinar
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
Nature Research Round Table: Maintenance of Human Pluripotent Stem Cells In Vitro
20:09
Webinar
Nature Research Round Table: Maintenance of Human Pluripotent Stem Cells In Vitro
Madeline Lancaster on Brain Organoids: Modeling Human Brain Development in a Dish
47:18
Webinar
Madeline Lancaster on Brain Organoids: Modeling Human Brain Development in a Dish
Human Pluripotent Stem Cells for the Treatment of Age-Related Macular Degeneration and Compliance Considerations for Clinical-Grade iPSCs
47:23
Webinar
Human Pluripotent Stem Cells for the Treatment of Age-Related Macular Degeneration and Compliance Co...
Whole Exome Sequencing Reveals Selective Pressures and Dominant Negative Mutations in hPSC Cultures
53:35
Webinar
Whole Exome Sequencing Reveals Selective Pressures and Dominant Negative Mutations in hPSC Cultures
Investigating Metabolic Disease with Human Pluripotent Stem Cells
49:25
Webinar
Investigating Metabolic Disease with Human Pluripotent Stem Cells
Nature Research Round Table: Human Pluripotent Stem Cell Lines as Disease Models
17:30
Webinar
Nature Research Round Table: Human Pluripotent Stem Cell Lines as Disease Models
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
50:49
Webinar
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
Maintaining and Assessing High-Quality hPSC Cultures
55:53
Webinar
Maintaining and Assessing High-Quality hPSC Cultures
Nature Research Round Table: Defining and Maintaining Pluripotency & hPSC Line Registration and Banking - Panel Discussion
33:47
Webinar
Nature Research Round Table: Defining and Maintaining Pluripotency & hPSC Line Registration and Bank...

Publications (1583)

iPSC-Based Modeling of RAG2 Severe Combined Immunodeficiency Reveals Multiple T Cell Developmental Arrests. M. Themeli et al. Stem cell reports 2020 feb

Abstract

RAG2 severe combined immune deficiency (RAG2-SCID) is a lethal disorder caused by the absence of functional T and B cells due to a differentiation block. Here, we generated induced pluripotent stem cells (iPSCs) from a RAG2-SCID patient to study the nature of the T cell developmental blockade. We observed a strongly reduced capacity to differentiate at every investigated stage of T cell development, from early CD7-CD5- to CD4+CD8+. The impaired differentiation was accompanied by an increase in CD7-CD56+CD33+ natural killer (NK) cell-like cells. T cell receptor D rearrangements were completely absent in RAG2SCID cells, whereas the rare T cell receptor B rearrangements were likely the result of illegitimate rearrangements. Repair of RAG2 restored the capacity to induce T cell receptor rearrangements, normalized T cell development, and corrected the NK cell-like phenotype. In conclusion, we succeeded in generating an iPSC-based RAG2-SCID model, which enabled the identification of previously unrecognized disorder-related T cell developmental roadblocks.
View publication
One-Stop Microfluidic Assembly of Human Brain Organoids To Model Prenatal Cannabis Exposure. Z. Ao et al. Analytical chemistry 2020

Abstract

Prenatal cannabis exposure (PCE) influences human brain development, but it is challenging to model PCE using animals and current cell culture techniques. Here, we developed a one-stop microfluidic platform to assemble and culture human cerebral organoids from human embryonic stem cells (hESC) to investigate the effect of PCE on early human brain development. By incorporating perfusable culture chambers, air-liquid interface, and one-stop protocol, this microfluidic platform can simplify the fabrication procedure and produce a large number of organoids (169 organoids per 3.5 cm × 3.5 cm device area) without fusion, as compared with conventional fabrication methods. These one-stop microfluidic assembled cerebral organoids not only recapitulate early human brain structure, biology, and electrophysiology but also have minimal size variation and hypoxia. Under on-chip exposure to the psychoactive cannabinoid, $\Delta$-9-tetrahydrocannabinol (THC), cerebral organoids exhibited reduced neuronal maturation, downregulation of cannabinoid receptor type 1 (CB1) receptors, and impaired neurite outgrowth. Moreover, transient on-chip THC treatment also decreased spontaneous firing in these organoids. This one-stop microfluidic technique enables a simple, scalable, and repeatable organoid culture method that can be used not only for human brain organoids but also for many other human organoids including liver, kidney, retina, and tumor organoids. This technology could be widely used in modeling brain and other organ development, developmental disorders, developmental pharmacology and toxicology, and drug screening.
View publication
IGF1-mediated human embryonic stem cell self-renewal recapitulates the embryonic niche. S. E. Wamaitha et al. Nature communications 2020

Abstract

Our understanding of the signalling pathways regulating early human development is limited, despite their fundamental biological importance. Here, we mine transcriptomics datasets to investigate signalling in the human embryo and identify expression for the insulin and insulin growth factor 1 (IGF1) receptors, along with IGF1 ligand. Consequently, we generate a minimal chemically-defined culture medium in which IGF1 together with Activin maintain self-renewal in the absence of fibroblast growth factor (FGF) signalling. Under these conditions, we derive several pluripotent stem cell lines that express pluripotency-associated genes, retain high viability and a normal karyotype, and can be genetically modified or differentiated into multiple cell lineages. We also identify active phosphoinositide 3-kinase (PI3K)/AKT/mTOR signalling in early human embryos, and in both primed and na{\{i}}ve pluripotent culture conditions. This demonstrates that signalling insights from human blastocysts can be used to define culture conditions that more closely recapitulate the embryonic niche."
View publication
View All Publications

Related Products

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Legal Statement:

This product was developed under license to intellectual property owned by WiCell™ Research Institute. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services) or clinical use (i.e., administration of this product or any material using this product to humans) or the right to implant any material made using this product into an animal by, or in collaboration with, a for-profit entity, for purposes other than basic pre-clinical research applications (including without limitation teratoma assays) to validate the function of the cells. Purchasers who do not agree to the terms and conditions set forth above should return the product in acceptable conditions to the seller for a refund.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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