How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free Media
Protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus
In this series of three Technical Videos, we will guide you through the protocol for transitioning human pluripotent stem cells (or hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus.
This series of technical videos will help ensure a successful transition of your hPSC lines into mTeSR™ Plus. We will use mTeSR™1 cultures transitioning to mTeSR™ Plus as an example for this video. If other feeder-free media are being used, some alterations to this protocol may be necessary.
Video 1: Plate Coating
Video 2: Generation of Cell Aggregates/Passaging
Video 3: Maintenance & Morphological Assessment
Learn more about mTeSR™ Plus hPSC maintenance media >
View a written protocol on how to transition hPSCs from feeder-free media to mTeSR™ Plus.
Publish Date:
May 20, 2020
Related Multimedia
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How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
Publish Date: May 20, 2020 -
How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
Publish Date: May 20, 2020 -
How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
Publish Date: May 20, 2020
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Healthy Control Human iPSC Line, Female, SCTi003-A
Human pluripotent stem cell line, frozen -
STEMdiff™ Trilineage Differentiation Kit
Functional assay kit to assess pluripotency by directed differentiation of human ES and iPS cells to all three germ layers -
cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells -
cGMP, enzyme-free human pluripotent stem cell selection and passaging reagent
