How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media such as mTeSR™1 into mTeSR™ Plus. This video will cover the generation of cell aggregates and passaging protocol.
0:00 - Intro
1:23 - ReLeSR™ Passaging
3:14 - GCDR Passaging
5:56 - Plate Setup for mTeSR Plus Transition
Watch the other videos in the series below:
Video 1: Plate Coating
Video 3: Maintenance and Morphological Assessment
Learn more about mTeSR™ Plus hPSC maintenance media >
Publish Date:
May 20, 2020
Related Multimedia
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How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free Media
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How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
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How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
Publish Date: May 20, 2020
Explore Our Products
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cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells -
cGMP, enzyme-free human pluripotent stem cell selection and passaging reagent -
STEMdiff™ Trilineage Differentiation Kit
Functional assay kit to assess pluripotency by directed differentiation of human ES and iPS cells to all three germ layers -
qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells
