How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells Cultured in mTeSR™ Plus
Protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus and will cover the generation of cell aggregates and passaging protocol
In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1, into mTeSR™ Plus.
This video will cover the generation of cell aggregates and describe a passaging protocol using ReLeSR™ or Gentle Cell Dissociation Reagent (GCDR).
Key topics covered in this video:
Video 1: Plate Coating for hPSC Cultures
Video 3: hPSC Maintenance and Morphological Assessment
This video will cover the generation of cell aggregates and describe a passaging protocol using ReLeSR™ or Gentle Cell Dissociation Reagent (GCDR).
Key topics covered in this video:
- • Assessing the quality of hPSC cultures through morphology using phase-contrast microscopy to determine when to passage
- • Passaging cell aggregates using ReLeSR™ or GCDR
- • Optimizing aggregate size for feeder-free PSC culture
- • Plating aggregates into maintenance medium
Video 1: Plate Coating for hPSC Cultures
Video 3: hPSC Maintenance and Morphological Assessment
Publish Date:
May 20, 2020
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