How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus

In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media such as mTeSR™1 into mTeSR™ Plus. This video will cover the generation of cell aggregates and passaging protocol.

0:00 - Intro
1:23 - ReLeSR™ Passaging
3:14 - GCDR Passaging
5:56 - Plate Setup for mTeSR Plus Transition

Watch the other videos in the series below:

Video 1: Plate Coating
Video 3: Maintenance and Morphological Assessment

Learn more about mTeSR™ Plus hPSC maintenance media >

See a written version of this protocol >

Publish Date: May 20, 2020 Views: 0