How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media such as mTeSR™1 into mTeSR™ Plus. This video will cover the generation of cell aggregates and passaging protocol.
0:00 - Intro
1:23 - ReLeSR™ Passaging
3:14 - GCDR Passaging
5:56 - Plate Setup for mTeSR Plus Transition
Watch the other videos in the series below:
Video 1: Plate Coating
Video 3: Maintenance and Morphological Assessment
Learn more about mTeSR™ Plus hPSC maintenance media >
0:00 - Intro
1:23 - ReLeSR™ Passaging
3:14 - GCDR Passaging
5:56 - Plate Setup for mTeSR Plus Transition
Watch the other videos in the series below:
Video 1: Plate Coating
Video 3: Maintenance and Morphological Assessment
Learn more about mTeSR™ Plus hPSC maintenance media >
Publish Date: May 20, 2020
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Explore Our Products
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STEMdiff™ Trilineage Differentiation Kit
Functional assay kit to assess pluripotency by directed differentiation of human ES and iPS cells to all three germ layers -
qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells
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cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells