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How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus

Protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus and will cover the generation of cell aggregates and passaging protocol

In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media such as mTeSR™1 into mTeSR™ Plus. This video will cover the generation of cell aggregates and passaging protocol. 0:00 - Intro 1:23 - ReLeSR™ Passaging 3:14 - GCDR Passaging 5:56 - Plate Setup for mTeSR Plus Transition Watch the other videos in the series below: Video 1: Plate Coating Video 3: Maintenance and Morphological Assessment Learn more about mTeSR™ Plus hPSC maintenance media >

See a written version of this protocol >

Publish Date: May 20, 2020

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