How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
Protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus and will cover the generation of cell aggregates and passaging protocol
In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media such as mTeSR™1 into mTeSR™ Plus. This video will cover the generation of cell aggregates and passaging protocol.
0:00 - Intro
1:23 - ReLeSR™ Passaging
3:14 - GCDR Passaging
5:56 - Plate Setup for mTeSR Plus Transition
Watch the other videos in the series below:
Video 1: Plate Coating
Video 3: Maintenance and Morphological Assessment
Learn more about mTeSR™ Plus hPSC maintenance media >
Publish Date:
May 20, 2020
Related Multimedia
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How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free Media
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How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
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How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
Publish Date: May 20, 2020
Explore Our Products
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STEMdiff™ Trilineage Differentiation Kit
Functional assay kit to assess pluripotency by directed differentiation of human ES and iPS cells to all three germ layers -
qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells -
cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells -
Healthy Control Human iPSC Line, Female, SCTi003-A
Human pluripotent stem cell line, frozen
