Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
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Developed and manufactured by Nucleus Biologics, Vitronectin XF™ is an effective alternative to Corning® Matrigel®. Use Vitronectin XF™ with mTeSR™1, mTeSR™ Plus, TeSR™-E8™, or TeSR™-AOF medium to provide a defined culture system for the maintenance of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells under feeder-free conditions. This system allows complete control over the culture environment, resulting in more consistent cell populations and reproducible results in downstream applications.
Note: CellAdhere™ Dilution Buffer and non-tissue culture-treated cultureware (e.g. Catalog #100-0096/27147) are required for use with Vitronectin XF™ and are available for purchase separately.
Figure 1. Morphology of Human ES and iPS Cells Cultured on Vitronectin XF™ Cell Culture Matrix in TeSR™-E8™
Undifferentiated human ES (H9) and iPS (WLS-1C) cell cultures exhibit normal morphology when cultured on Vitronectin XF™. Colonies are round, tightly packed and multilayered, with a high nucleus-to-cytoplasm ratio. Cells were transferred directly from Matrigel® hESC-Qualified Matrix without an adaptation step. Note: Colonies grown in TeSR™-E8™ have a more condensed and round morphology when grown on Vitronectin XF™ matrix, compared to colonies grown on Matrigel® hESC-Qualified Matrix, which are more diffuse and irregularly shaped.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (11)
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Vitronectin XF is developed and manufactured by Nucleus Biologics, and Vitronectin XF™ is a trademark of Nucleus Biologics.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.