hPSC-Derived Endoderm Cell Research

After generating definitive endoderm (DE) cells from human pluripotent stem cells (hPSCs), the cells can be evaluated prior to downstream differentiation to hepatic, intestinal, pancreatic or pulmonary cells. See MoreIdentify and characterize your DE cells with high-quality anti-CXCR4 and column-free immunomagnetic cell isolation kits to achieve DE cell purity levels of up to 99%.

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Video (3:02) Fast and Easy Cell Isolation with EasySep™
Scientific Poster Highly Efficient Differentiation of Human Pluripotent Stem Cells to Multipotent Definitive Endoderm Using the Serum-free and Animal Component-free STEMdiff™ Definitive Endoderm Culture System

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Products

Cell Culture Media and Supplements (4 1)

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STEMdiff™ Gastric Organoid Differentiation Kit

Culture medium kit for differentiation of human gastric organoids

Antibodies (4 10)

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Anti-Mouse OCT4 (OCT3) Antibody, Clone 40

Mouse monoclonal IgG1 antibody against human, mouse OCT4 (OCT3)
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Anti-Human CD184 (CXCR4) Antibody, Clone 12G5

Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD184 (CXCR4)
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Anti-Human CD117 (c-Kit) Antibody, Clone 104D2

Mouse monoclonal IgG1 antibody against human, cynomolgus, cow CD117 (c-Kit)
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Anti-Human SSEA-4 Antibody, Clone MC-813-70

Mouse monoclonal IgG3 antibody against human, mouse, rat SSEA-4

Buffers and Solutions (4 4)

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EasySep™ Buffer

Cell separation buffer
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RoboSep™ Buffer

Cell separation buffer
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RoboSep™ Buffer 2

Cell separation buffer
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D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

Cell Dyes and Detection Assay Kits (4 7)

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GloCell™ Fixable Viability Dye Red 780

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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DAPI (Hydrochloride)

DNA-labeling dye
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GloCell™ Fixable Viability Dye Violet 540

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 510

Amine-labeling fluorescent dye for live/dead staining of mammalian cells

Cultureware and General Supplies (4 1)

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Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)

Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)

Genome Editing and Molecular Tools (4 3)

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Human Pluripotent Stem Cell-Derived Endoderm qPCR Array

For characterizaton of hPSC-derived progenitor cells of definitive endoderm, hepatic, intestinal, or pancreatic lineages
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qPCR Master Mix Kit

For probe-based assays and arrays
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Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array

For characterization of hPSC trilineage differentiation to endoderm, mesoderm, and ectoderm germ layers

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