hPSC-Derived Endoderm Cell Research
Human pluripotent stem cells (hPSCs) have the ability to differentiate to cells of all three germ layers: endoderm, ectoderm and mesoderm.
Products
Cell Culture Media and Supplements 4 (10)
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DMEM/F-12 with 15 mM HEPES
Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer -
STEMdiff™ Gastric Organoid Differentiation Kit
Culture medium kit for differentiation of human gastric organoids -
STEMdiff™ Lung Progenitor Kit
Serum-free medium for efficient and reproducible generation of lung progenitor cells from human ES and iPS cells -
STEMdiff™ Definitive Endoderm Kit
Defined animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm
Tissue and Cell Culture Dissociation Reagents 4 (1)
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Gentle Cell Dissociation Reagent
cGMP, enzyme-free cell dissociation reagent
Matrices and Substrates 4 (1)
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Vitronectin XF™
Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
Cytokines and Proteins 4 (13)
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Human Recombinant BMP-4
Bone morphogenetic protein 4 -
Human Recombinant FGF-7, ACF
Fibroblast growth factor 7, animal component-free -
Human Recombinant FGF-10 (KGF-2), ACF
Fibroblast growth factor 10, animal component-free -
Human/Mouse Recombinant BMP-2, ACF
Bone morphogenetic protein 2, animal component-free
Small Molecules 4 (5)
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Sodium Butyrate
Epigenetic modifier; Inhibits histone deacetylase -
CHIR99021
WNT pathway activator; Inhibits GSK3 -
Y-27632 (Dihydrochloride)
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2 -
IDE1
Activin/BMP/TGF-β pathway activator
Antibodies 4 (1)
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Anti-Human SSEA-5 Antibody, Clone 8e11
Mouse monoclonal IgG1 antibody against human SSEA-5
Buffers and Solutions 4 (1)
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D-PBS (Without Ca++ and Mg++)
Dulbecco’s phosphate-buffered saline without calcium and magnesium