hPSC-Derived Endoderm Cell Research
Human pluripotent stem cells (hPSCs) have the ability to differentiate into cells of all three germ layers: endoderm, ectoderm, and mesoderm. hPSCs directed toward the endoderm lineage can be further differentiated into cells of the digestive and respiratory tracts, including lung, liver, pancreas, and intestine. STEMdiff™ is a line of culture medium kits specifically optimized for hPSC differentiation—to reproducibly differentiate multiple human embryonic stem (ES) and induced pluripotent stem (iPS) cell lines to 2D cell types and 3D organoid models originating from all three embryonic germ layers. Use our new interactive STEMdiff™ product explorer to find specific differentiation kits for your tissue of interest.
Products
Cell Culture Media and Supplements 4 (10)
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STEMdiff™ Intestinal Organoid Growth Medium
Serum-free medium for long-term maintenance of hPSC-derived human intestinal organoids -
STEMdiff™ Intestinal Organoid Kit
Serum-free medium kit for establishment and maintenance of human intestinal organoids from hPSCs -
STEMdiff™ Pancreatic Progenitor Kit
Serum-free medium for the differentiation of human ES and iPS<br /> cells to pancreatic progenitor cells -
STEMdiff™ Lung Progenitor Kit
Serum-free medium for efficient and reproducible generation of lung progenitor cells from human ES and iPS cells
Tissue and Cell Culture Dissociation Reagents 4 (1)
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Gentle Cell Dissociation Reagent
cGMP, enzyme-free cell dissociation reagent
Matrices and Substrates 4 (2)
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CellAdhere™ Dilution Buffer
Dilution buffer for matrix proteins, e.g. Vitronectin XF™ -
Vitronectin XF™
Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
Cytokines and Proteins 4 (13)
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Human Recombinant BMP-4
Bone morphogenetic protein 4 -
Human Recombinant FGF-7, ACF
Fibroblast growth factor 7, animal component-free -
Human Recombinant FGF-10 (KGF-2), ACF
Fibroblast growth factor 10, animal component-free -
Human/Mouse Recombinant BMP-2, ACF
Bone morphogenetic protein 2, animal component-free
Small Molecules 4 (5)
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Sodium Butyrate
Epigenetic modifier; Inhibits histone deacetylase -
CHIR99021
WNT pathway activator; Inhibits GSK3 -
Y-27632 (Dihydrochloride)
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2 -
IDE1
Activin/BMP/TGF-β pathway activator
Antibodies 4 (1)
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Anti-Human SSEA-5 Antibody, Clone 8e11
Mouse monoclonal IgG1 antibody against human SSEA-5
Buffers and Solutions 4 (1)
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D-PBS (Without Ca++ and Mg++)
Dulbecco’s phosphate-buffered saline without calcium and magnesium
