Anti-Human SSEA-5 Antibody, Clone 8e11

Mouse monoclonal IgG1 antibody against human SSEA-5

Anti-Human SSEA-5 Antibody, Clone 8e11

Mouse monoclonal IgG1 antibody against human SSEA-5

From: 178 USD
Catalog #
60063_C
Mouse monoclonal IgG1 antibody against human SSEA-5

Overview

The 8e11 antibody reacts with stage-specific embryonic antigen-5 (SSEA-5), a terminal H type-1 glycan (Fuc1-2Galβ1-3GlcNAcβ1) expressed on the surface of cells in the inner cell mass of the human blastocyst during embryogenesis, and on undifferentiated human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Expression of SSEA-5 is rapidly down-regulated upon cellular differentiation. The 8e11 antibody can be used in conjunction with other pluripotency surface markers such as CD9 and CD90, or CD50 and CD200, to sort and remove undifferentiated teratoma-forming cells from incompletely differentiated stem cell cultures.
Subtype
Primary Antibodies
Target Antigen
SSEA-5
Alternative Names
Stage-specific embryonic antigen-5
Reactive Species
Human
Conjugation
Alexa Fluor 488, APC, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Stem Cell Biology
Clone
8e11
Gene ID
Not applicable
Isotype
IgG1, kappa

Data Figures

Data for FITC-Conjugated

Figure 1. Data for FITC-Conjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Labeling of human ES cells with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram).
(B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC.
(C) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (lane 2) with Anti-Human SSEA-5 Antibody, Clone 8e11.

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of human embryonic stem (ES) cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Labeling of human ES cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).
(B) Flow cytometry analysis of human induced pluripotent stem (iPS) cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Labeling of human iPS cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).
(C) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Inset shows labeling of human ES cells with a mouse IgG1, kappa PE isotype control antibody.
(D) Human iPS cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Inset shows labeling of human iPS cells with a mouse IgG1, kappa PE isotype control antibody.

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Labeling of human ES cells with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC. (C) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (lane 2) with Anti-Human SSEA-5 Antibody, Clone 8e11.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60063, 60063.1
Lot #
All
Language
English
Catalog #
60063AZ, 60063AZ.1
Lot #
All
Language
English
Catalog #
60063FI, 60063FI.1
Lot #
All
Language
English
Catalog #
60063PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60063, 60063.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60063AZ, 60063AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60063FI, 60063FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60063PE.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

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