Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20
Mouse monoclonal IgG2b antibody against human OCT4 (OCT3)
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Overview
Data Figures

Figure 1. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of ES cells cultured with mTeSR™1 on Corning® Matrigel®. The ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488. Labeling of the ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (Catalog #60072AD) is shown (solid line histogram). (B) Human ES cells were cultured with TeSR™-E8™ on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488. Inset shows labeling of human ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488. (C) DAPI counterstaining of the cells shown in figure (B); nuclear localization of the OCT4 (OCT3) marker is evident.

Figure 2. Data for PE-Conjugated
(A) Flow cytometry analysis of human ES cells cultured with mTeSR™1 on Corning® Matrigel®. The ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE. Labeling of the ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (Catalog #60072PE) is shown (solid line histogram). (B) Human ES cells were cultured with TeSR™-E8™ on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE. Inset shows labeling of human ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE. (C) DAPI counterstaining of the cells shown in figure (B); nuclear localization of the OCT4 (OCT3) marker is evident.

Figure 3. Data for Unconjugated
(A) Human induced pluripotent stem (iPS) cells were cultured with TeSR™-E8™ on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and stained with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC.
(B) Flow cytometry analysis of human embryonic stem (ES) cells cultured with mTeSR™1 on Corning® Matrigel®. The ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, followed by goat anti-mouse IgG, FITC. Labeling of the ES cells with a mouse IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram). (C) Flow cytometry analysis of human iPS cells cultured with TeSR™-E8™ on Vitronectin XF™. The cells were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, followed by goat anti-mouse IgG, FITC (filled histogram) or a mouse IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC (solid line histogram). (D) Western blot analysis of denatured/reduced cell lysates with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20. Lane 1, human ES cells cultured with mTeSR™1 on Corning® Matrigel®, lane 2 (negative control), mouse E13.5 neural progenitor cells cultured with NeuroCult™ Proliferation Kit (Mouse, Catalog #05702).
Protocols and Documentation
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Applications
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Resources and Publications
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Abstract
Abstract
Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.