hPSC-Derived Endoderm Cell Research

Differentiate human pluripotent stem cells (hPSCs) into cells of the endoderm lineage with our specialized cell culture media. STEMdiff™ is a line of culture medium kits specifically optimized for hPSC differentiation—to reproducibly differentiate multiple human embryonic stem (ES) and induced pluripotent stem (iPS) cell lines to 2D cell types and 3D organoid models originating from all three embryonic germ layers. Use our new interactive STEMdiff™ product explorer to find specific differentiation kits for your tissue of interest. Further characterize the downstream cells with our high-quality verified antibodies. See More

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Webinar (50:33) Understanding Pancreatic Development and Diabetes Using Patient-Specific iPS Cells
Webinar (36:59) Derivation of Metabolically Active Hepatocytes from Human Pluripotent Stem Cells
Webinar (50:32) Understanding Pancreatic Development and Diabetes Using Patient-Specific iPS Cells
Scientific Poster Highly Efficient and Reproducible Differentiation of hPSCs to PDX1+/NKX6.1+ Pancreatic Progenitors

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Products

Cell Culture Media and Supplements 4 (16)

STEMdiff™ Branching Lung Organoid Maturation Kit

Serum-free medium for maturation of hPSC-derived branching lung organoids
STEMdiff™ Branching Lung Organoid Kit

Serum-free media for generation and maturation of branching lung organoids from human ES and iPS cells
STEMdiff™ Intestinal Organoid Growth Medium

Serum-free medium for long-term maintenance of hPSC-derived human intestinal organoids
STEMdiff™ Intestinal Organoid Kit

Serum-free medium kit for establishment and maintenance of human intestinal organoids from hPSCs

Tissue and Cell Culture Dissociation Reagents 4 (1)

Gentle Cell Dissociation Reagent

cGMP, enzyme-free cell dissociation reagent

Matrices and Substrates 4 (2)

CellAdhere™ Dilution Buffer

Dilution buffer for matrix proteins, e.g. Vitronectin XF™
Vitronectin XF™

Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions

Cytokines and Proteins 4 (21)

Human Recombinant BMP-4

Bone morphogenetic protein 4
Human Recombinant BMP-2

Bone morphogenetic protein 2
Human Recombinant DKK-1

Dickkopf-related protein 1
Human Recombinant FGF-7, ACF

Fibroblast growth factor 7, animal component-free

Small Molecules 4 (5)

Sodium Butyrate

Epigenetic modifier; Inhibits histone deacetylase
LDN193189

BMP pathway inhibitor; Inhibits ALK1, ALK2, ALK3, and ALK6
IWR-1-endo

WNT pathway inhibitor; AXIN2 stabilizer
(-)-Indolactam V

Protein kinase C (PKC) activator

Antibodies 4 (10)

Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J

Mouse monoclonal IgG1 antibody against human, chimpanzee, gibbon TRA-2-54 (tissue non-specific alkaline phosphatase)
Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E

Mouse monoclonal IgG1 antibody against human, chimpanzee, gibbon TRA-2-49 (tissue non-specific alkaline phosphatase)
Anti-Mouse OCT4 (OCT3) Antibody, Clone 40

Mouse monoclonal IgG1 antibody against human, mouse OCT4 (OCT3)
Anti-Human CD184 (CXCR4) Antibody, Clone 12G5

Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD184 (CXCR4)

Buffers and Solutions 4 (1)

D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

Cultureware and General Supplies 4 (1)

Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)

Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)

Genome Editing and Molecular Tools 4 (2)

qPCR Master Mix Kit

For probe-based assays and arrays
Human Pluripotent Stem Cell-Derived Endoderm qPCR Array

For characterizaton of hPSC-derived progenitor cells of definitive endoderm, hepatic, intestinal, or pancreatic lineages