hPSC-Derived Endoderm Cell Research

Differentiate human pluripotent stem cells (hPSCs) to cells of the endoderm lineage with our specialized cell culture media. See MoreUse our definitive endoderm (DE) differentiation kit to obtain functional DE cells that are capable of downstream differentiation to hepatic, intestinal, pancreatic and pulmonary cells. To directly differentiate to pancreatic cells, use our pancreatic progenitor kit to generate functional PDX1⁺/NKX6.1⁺ cells that can be matured into insulin-producing beta cells. Further characterize the downstream cells with our high-quality verified antibodies .

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Webinar (50:33) Understanding Pancreatic Development and Diabetes Using Patient-Specific iPS Cells
Webinar (36:59) Derivation of Metabolically Active Hepatocytes from Human Pluripotent Stem Cells
Webinar (50:32) Understanding Pancreatic Development and Diabetes Using Patient-Specific iPS Cells
Scientific Poster Highly Efficient and Reproducible Differentiation of hPSCs to PDX1+/NKX6.1+ Pancreatic Progenitors

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Products

Cell Culture Media and Supplements 4 (6)

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NeuroCult™ SM1 Without Insulin

Serum-free neural supplement without insulin
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NeuroCult™ SM1 Without Antioxidants

Serum-free neural supplement without antioxidants
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N2 Supplement-A

For neural and pancreatic differentiation of mouse and human ES and iPS cells
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NeuroCult™ SM1 Without Vitamin A

Supplement (50X) without vitamin A for the culture of neural progenitor cells

Tissue and Cell Culture Dissociation Reagents 4 (1)

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Gentle Cell Dissociation Reagent

cGMP, enzyme-free cell dissociation reagent

Cytokines and Proteins 4 (3)

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Human/Mouse Recombinant BMP-2, ACF

Bone morphogenetic protein 2, animal component-free
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Human/Mouse Recombinant Activin A, ACF

Activin A, animal component-free
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Human/Mouse Recombinant Activin A

Activin A

Small Molecules 4 (5)

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Sodium Butyrate

Epigenetic modifier; Inhibits histone deacetylase
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LDN193189

BMP pathway inhibitor; Inhibits ALK1, ALK2, ALK3, and ALK6
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IWR-1-endo

WNT pathway inhibitor; AXIN2 stabilizer
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(-)-Indolactam V

Protein kinase C (PKC) activator

Antibodies 4 (2)

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Anti-Mouse OCT4 (OCT3) Antibody, Clone 40

Mouse monoclonal IgG1 antibody against human, mouse OCT4 (OCT3)
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Anti-Human SSEA-4 Antibody, Clone MC-813-70

Mouse monoclonal IgG3 antibody against human, mouse, rat SSEA-4

Buffers and Solutions 4 (1)

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D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

Cultureware and General Supplies 4 (1)

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Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)

Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)

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