hPSC-Derived Mesoderm Cell Research

Early mesoderm cells can be further differentiated to specialized cell types, such as mesenchymal stem cells (MSCs) and their derivatives (osteoblasts, chondrocytes, adipocytes) and endothelial cells. See MoreCells can be transferred to downstream differentiation conditions as early as 2 - 4 days after induction, depending on the desired cell type and differentiation protocol. For differentiation to MSCs and MSC-derived cells, use our specialized animal component-free media and associated protocols for MSC expansion and differentiation.

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Products

Cell Culture Media and Supplements (4 12)

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STEMdiff™ Blood Vessel Organoid Kit

Media and supplements for differentiation of human pluripotent stem cells to blood vessel organoids
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STEMdiff™ Endothelial Expansion Medium Kit

Expand human pluripotent stem cell-derived endothelial cells
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STEMdiff™ Endothelial Differentiation Kit

Efficient differentiation of human pluripotent stem cells to endothelial cells
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STEMdiff™ APEL™2-LI Medium

Defined, low-insulin, animal component-free medium for the differentiation of human ES and iPS cells

Small Molecules (4 10)

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SB431542 (Hydrate)

Activin/BMP/TGF-β pathway inhibitor; Inhibits ALK4, ALK5, and ALK7
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A 83-01

Activin/NODAL/TGF-β pathway inhibitor; Inhibits ALK5, ALK4, and ALK7
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SB202190

p38 MAPK inhibitor
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KY02111

WNT pathway inhibitor

Cytokines and Proteins (4 53)

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Human Recombinant PDGF-DD

Platelet-derived growth factor DD
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Human Recombinant RANKL

Receptor activator of nuclear factor kappa-B ligand
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Human Recombinant R-Spondin-1

R-Spondin-1
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Human Recombinant BMP-4

Bone morphogenetic protein 4

Antibodies (4 6)

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Anti-Human CD45 Antibody, Clone 2D1

Mouse monoclonal IgG1 antibody against human CD45
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Anti-Human CD326 (EpCAM) Antibody, Clone 323/A3

Mouse monoclonal IgG1 antibody against human CD326 (EpCAM)
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Anti-Human CD34 Antibody, Clone 8G12

Mouse monoclonal IgG1 antibody against human CD34
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Anti-Human CD45 Antibody, Clone HI30

Mouse monoclonal IgG1 antibody against human, chimpanzee CD45

Matrices and Substrates (4 7)

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MesenCult™-SF Attachment Substrate

Serum-free attachment substrate for human mesenchymal stem cells
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Vitronectin XF™

Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
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CellAdhere™ Vitronectin, Human, Solution

Purified human vitronectin solution for tissue engineering research and cell culture
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0.1% Gelatin in Water

Coating for cultureware

Tissue and Cell Culture Dissociation Reagents (4 2)

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ACCUTASE™

Cell detachment solution
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Animal Component-Free Cell Dissociation Kit

Dissociation kit for human stem and progenitor cells

Cell Storage Media (4 1)

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MesenCult™-ACF Freezing Medium

Animal component-free MSC cryopreservation medium

Cell Dyes and Detection Assay Kits (4 6)

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GloCell™ Fixable Viability Dye Violet 540

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 510

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye UV 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells

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