Human Recombinant G-CSF (HEK293-expressed)

Granulocyte colony-stimulating factor, Fc tag

Human Recombinant G-CSF (HEK293-expressed)

Granulocyte colony-stimulating factor, Fc tag

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Granulocyte colony-stimulating factor, Fc tag
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Overview

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf & Nicola. J Cell Physiol, 1983). G-CSF causes a range of effects including a transient reduction of SDF-1 expression (Petit et al. Nat Immunol, 2002), the activation of metalloproteases that cleave VCAM-1 (Levesque et al. Blood, 2001), and the release of norepinephrine from the sympathetic nervous system (Katayama et al. Cell, 2006), leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. Binding of G-CSF to its receptor leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways. At the carboxy terminus, Human Recombinant G-CSF (HEK293-expressed) contains a human IgG1 Fc tag.
Subtype
Cytokines
Alternative Names
Colony-stimulating factor 3, CSF-3, MGI-1G, Pluripoietin
Cell Type
Cancer Cells and Cell Lines, Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Neurons
Species
Human
Area of Interest
Neuroscience, Stem Cell Biology
Purity
≥ 94%

Data Figures

Figure 1. Biological Activity and Molecular Mass of Human Recombinant G-CSF (HEK293-expressed)

(A) The biological activity of Human Recombinant G-CSF (HEK293-expressed) was tested by its ability to promote the proliferation of NFS-60 cells. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 for this effect is ≤ 800 pg/mL. (B) Human Recombinant G-CSF (HEK293-expressed) was resolved with SDS-PAGE under reducing conditions. Human Recombinant G-CSF has a predicted molecular mass of 45.4 kDa and migrates with an apparent molecular mass of 48 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1718, 100-1719
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1718
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1719
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.