Human Pluripotent Stem Cell-Derived Endoderm qPCR Array

For characterizaton of hPSC-derived progenitor cells of definitive endoderm, hepatic, intestinal, or pancreatic lineages

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Human Pluripotent Stem Cell-Derived Endoderm qPCR Array

For characterizaton of hPSC-derived progenitor cells of definitive endoderm, hepatic, intestinal, or pancreatic lineages

From: 205 USD
Catalog #
07531_C
For characterizaton of hPSC-derived progenitor cells of definitive endoderm, hepatic, intestinal, or pancreatic lineages

What's Included

  • Human Pluripotent Stem Cell-Derived Endoderm qPCR Array (Catalog #07531)
    • hPSC-Derived Endoderm qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Pluripotent Stem Cell-Derived Endoderm qPCR Array (Catalog #07532)
    • hPSC-Derived Endoderm qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Pluripotent Stem Cell-Derived Endoderm qPCR Array (Catalog #07533)
    • hPSC-Derived Endoderm qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Pluripotent Stem Cell-Derived Endoderm qPCR Array (Catalog #07534)
    • hPSC-Derived Endoderm qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

The Human Pluripotent Stem Cell (hPSC)-Derived Endoderm Quantitative Polymerase Chain Reaction (qPCR) Array is designed for characterization of hPSC-derived definitive endodermal progenitor cells and their differentiated progeny including pancreatic, hepatic, and intestinal cells. This qPCR array provides the gene expression profile of hPSC-derived endodermal cells following in vitro differentiation, and can aid in development of protocols for directed differentiation towards endodermal lineages. Genes were selected based on their demonstrated expression in hPSCs, or in hPSC-derived ectodermal, mesodermal, and endodermal lineage cells (D’Amour et al.), including pancreatic (Kroon et al.), hepatic (Hay et al.), and intestinal progenitor cells (Grapin-Botton & Melton).

qPCR is used to determine changes in steady-state mRNA levels of gene expression across multiple samples, generally normalized to the relative expression of internal control genes. Gene-specific primers amplify target sequences within cDNA pools reverse-transcribed from mRNA and, as with TaqMan® technology, hybridized sequence-specific probes provide a fluorescent signal from the 5' exonuclease activity of Taq DNA polymerase. The accumulation rate of the fluorescent signal is used to quantify the sample cDNA, and thereby the amount of mRNA in the original cell lysate.

This qPCR array contains validated primers and probes for detection of 90 genes whose expression is correlated with hPSCs or hPSC-derived ectodermal, mesodermal, and endodermal cells, or pancreatic, hepatic, and intestinal progenitor cells, as well as six endogenous (housekeeping) control genes. TBP (TATA box-binding protein) qPCR Control Template is provided as a synthetic DNA positive control. Additional gene information and a qPCR analysis tool are available at www.stemcell.com/qPCRanalysis
Cell Type
Endoderm, PSC-Derived, Hepatic Cells, Intestinal Cells, Pancreatic Cells, Pluripotent Stem Cells
Species
Human
Area of Interest
Stem Cell Biology

Data Figures

Figure 1. Human Pluripotent Stem Cell-Derived Endoderm qPCR Array Plate Configuration

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
07531, 07533
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
07531, 07533
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
07531
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
07531, 07533
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
07531, 07533
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Catalog #
07531, 07533
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
07533
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (1)

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