Total RNA Purification Kit

For purification of RNA from cells
Total RNA Purification Kit

For purification of RNA from cells

50 Preps
Catalog # 79040
323 EUR
Required Products
  1. Filtered Pipette Tips, 1000 µL
    Corning® Filtered Pipette Tips

    Sterile, polypropylene filtered pipette tips

  2. Costar® Microcentrifuge Tubes, 0.65 mL
    Costar® Microcentrifuge Tubes

    Non-sterile, clear, polypropylene snap-cap microcentifuge tubes

Overview

The Total RNA Purification Kit uses a membrane-based system to quickly purify intact total RNA from 1 x 10^2 to 5 x 10^6 cells without an organic extraction step, eliminating the need for additional purification steps. The Total RNA Purification Kit includes an on-column DNase I treatment to significantly reduce genomic DNA contamination and ensure the purified RNA is suitable for sensitive downstream applications. Intact total RNA can be isolated in as little as 30 minutes. The Total RNA Purification Kit is not recommended for the purification of small RNAs (< 200 nt), such as miRNA.
Components
  • Total RNA Purification Kit (Catalog #79040)
    • RNA Minicolumns, 50x
    • Collection Tubes, 2 x 50
    • Column Wash Solution, 5 mL
    • 1-Thioglycerol, 900 µL
    • DNase I (lyophilized), 1 x 1 vial
    • Nuclease-Free Water, 13 mL
    • MnCl2, 250 µL
    • RNA Lysis Buffer, 32.5 mL
    • Elution Tubes, 50x
    • RNA Wash Solution, 35 mL
    • DNA Digestion Buffer, 2.5 mL

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Total RNA Purification Kit
Catalog #
79040
Lot #
All
Language
English
Document Type
Manual
Product Name
Total RNA Purification Kit
Catalog #
79040
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Total RNA Purification Kit
Catalog #
79040
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Total RNA Purification Kit
Catalog #
79040
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Total RNA Purification Kit
Catalog #
79040
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Total RNA Purification Kit
Catalog #
79040
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Total RNA Purification Kit
Catalog #
79040
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
Total RNA Purification Kit
Catalog #
79040
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
Total RNA Purification Kit
Catalog #
79040
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Product Name
Total RNA Purification Kit
Catalog #
79040
Lot #
All
Language
English

Educational Materials (2)

Brochure
qPCR Arrays for Cell Characterization
Technical Bulletin
Protocol for Total RNA Isolation from Cells

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