Mouse Pluripotent Stem Cell Research
Many protocols to differentiate mouse iPS cells and ES cells require the formation of embryoid bodies (EBs) followed by subculture in lineage-specific differentiation conditions.
Products
Cell Culture Media and Supplements 4 (4)
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N2 Supplement-B
For neural differentiation of mouse and human ES and iPS cells -
MEM Non-Essential Amino Acid Solution (100X)
MEM non-essential amino acid solution (100X) -
L-Glutamine
Cell culture supplement -
DMEM with 4500 mg/L D-Glucose
Dulbecco's Modified Eagle's Medium (DMEM) with 4500 mg/L D-glucose
Matrices and Substrates 4 (3)
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0.1% Gelatin in Water
Coating for cultureware -
CellAdhere™ Dilution Buffer
Dilution buffer for matrix proteins, e.g. Vitronectin XF™ -
Vitronectin XF™
Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
Tissue and Cell Culture Dissociation Reagents 4 (1)
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Gentle Cell Dissociation Reagent
cGMP, enzyme-free cell dissociation reagent
Small Molecules 4 (24)
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Nicotinamide
Cell culture supplement for differentiation of ES and iPS cells -
TTNPB
Retinoid pathway activator; Activates retinoic acid receptor (RAR) -
LY364947
Activin/BMP/TGF-β pathway inhibitor; Inhibits ALK5 -
KY02111
WNT pathway inhibitor
Cytokines and Proteins 4 (7)
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Mouse Recombinant SCF (P. pastoris-expressed)
Stem cell factor -
Human Recombinant BMP-4, ACF
Bone morphogenetic protein 4, animal component-free -
Human/Mouse/Rat Recombinant Follistatin-Resistant Activin A, ACF
Follistatin-resistant Activin A, animal component-free -
Human Recombinant BMP-4
Bone morphogenetic protein 4
Cultureware and General Supplies 4 (2)
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AggreWell™400
Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids -
AggreWell™800
Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids
Buffers and Solutions 4 (1)
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D-PBS (Without Ca++ and Mg++)
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Primary and Cultured Cells 4 (1)
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Human Peripheral Blood-Derived M1 Macrophages, SF-Cultured, Frozen
Primary human cells, frozen
Cell Dyes and Detection Assay Kits 4 (6)
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GloCell™ Fixable Viability Dye Violet 540
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye Violet 510
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye Violet 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye UV 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells