Mouse Pluripotent Stem Cell Research
Many protocols to differentiate mouse iPS cells and ES cells require the formation of embryoid bodies (EBs) followed by subculture in lineage-specific differentiation conditions.
Products
Cell Culture Media and Supplements (4 5)
-
ES-Cult™ M3120
Base methylcellulose medium for in vitro differentiation of mouse ES or iPS cells to hematopoietic or endothelial progenitor cells -
DMEM with 4500 mg/L D-Glucose
Dulbecco's Modified Eagle's Medium (DMEM) with 4500 mg/L D-glucose -
N2 Supplement-B
For neural differentiation of mouse and human ES and iPS cells -
MEM Non-Essential Amino Acid Solution (100X)
MEM non-essential amino acid solution (100X)
Tissue and Cell Culture Dissociation Reagents (4 1)
-
Gentle Cell Dissociation Reagent
cGMP, enzyme-free cell dissociation reagent
Small Molecules (4 25)
-
SB431542
Activin/BMP/TGF-β pathway inhibitor; Inhibits ALK4, ALK5, and ALK7 -
Dorsomorphin
BMP and AMPK pathway inhibitor; Inhibits ALK2, ALK3, ALK6, and AMPK -
Nicotinamide
Cell culture supplement for differentiation of ES and iPS cells -
XAV939
WNT pathway inhibitor; Inhibits TNKS1 and TNKS2
Cytokines and Proteins (4 3)
-
Human/Mouse Recombinant Activin A, ACF
Activin A, animal component-free -
Mouse Recombinant SCF
Stem cell factor -
Human/Mouse Recombinant Activin A
Activin A
Cultureware and General Supplies (4 2)
-
AggreWell™400
Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids -
AggreWell™800
Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids
Buffers and Solutions (4 1)
-
D-PBS (Without Ca++ and Mg++)
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Cell Dyes and Detection Assay Kits (4 6)
-
GloCell™ Fixable Viability Dye Violet 540
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye Violet 510
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye Violet 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye UV 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells