Mouse Pluripotent Stem Cell Research

Many protocols to differentiate mouse iPS cells and ES cells require the formation of embryoid bodies (EBs) followed by subculture in lineage-specific differentiation conditions. See MoreOur specialized microwell plates allow users to control the size and shape of the EBs allowing for reproducibility, optimization of differentiation protocols to specific lineages, standardization of the production of EBs and reduction of variability associated with mouse iPS cell and ES cell differentiation. Reagents and protocols suitable for differentiation towards hematopoietic, neural, and endothelial cell types are also available.

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Brochure Standardized solutions for mouse pluripotent stem cells
Wallchart Pluripotent cell isolation for regenerative medicine

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Products

Cell Culture Media and Supplements (4 5)

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ES-Cult™ M3120

Base methylcellulose medium for in vitro differentiation of mouse ES or iPS cells to hematopoietic or endothelial progenitor cells
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DMEM with 4500 mg/L D-Glucose

Dulbecco's Modified Eagle's Medium (DMEM) with 4500 mg/L D-glucose
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N2 Supplement-B

For neural differentiation of mouse and human ES and iPS cells
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MEM Non-Essential Amino Acid Solution (100X)

MEM non-essential amino acid solution (100X)

Matrices and Substrates (4 1)

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0.1% Gelatin in Water

Coating for cultureware

Tissue and Cell Culture Dissociation Reagents (4 1)

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Gentle Cell Dissociation Reagent

cGMP, enzyme-free cell dissociation reagent

Small Molecules (4 25)

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DAPT

Notch pathway inhibitor; Inhibits γ-secretase
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DMH1

Activin/Nodal/TGFβ pathway inhibitor; Inhibits ALK2
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SB431542 (Hydrate)

Activin/BMP/TGF-β pathway inhibitor; Inhibits ALK4, ALK5, and ALK7
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Dorsomorphin

BMP and AMPK pathway inhibitor; Inhibits ALK2, ALK3, ALK6, and AMPK

Cytokines and Proteins (4 3)

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Human/Mouse Recombinant Activin A, ACF

Activin A, animal component-free
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Mouse Recombinant SCF

Stem cell factor
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Human/Mouse Recombinant Activin A

Activin A

Cultureware and General Supplies (4 2)

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AggreWell™400

Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids
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AggreWell™800

Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids

Buffers and Solutions (4 1)

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D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

Cell Dyes and Detection Assay Kits (4 6)

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GloCell™ Fixable Viability Dye Violet 540

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 510

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye UV 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells

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