GloCell™ Fixable Viability Dye Violet 510

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
Catalog #
75010_C
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
From: 94 USD
Required Products
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

  2. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

GloCell™ Fixable Viability Dye Violet 510 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.


GloCell™ Fixable Viability Dye Violet 510 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 510 nm that can be detected using the 525/50 band pass filter used to detect AmCyan.
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Flow Cytometry, Immunofluorescence
Brand
GloCell
Area of Interest
Angiogenic Cell Research, Antibody Development, Cancer, Cell Line Development, Cell Therapy, Chimerism, Disease Modeling, Drug Discovery and Toxicity Testing, Endothelial Cell Biology, Epithelial Cell Biology, HIV, HLA, Hybridoma Generation, Immunology, Infectious Diseases, Metabolism, Mitosis, Neuroscience, Organoids, Respiratory Research, Stem Cell Biology, Transplantation Research

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
GloCell™ Fixable Viability Dye Violet 510
Catalog #
75010, 75010.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
GloCell™ Fixable Viability Dye Violet 510
Catalog #
75010, 75010.1
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Data and Publications

Data

Figure 1.

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Violet 510. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Violet 510.

Figure 2.

Excitation/Emission spectra for GloCell™ Fixable Viability Dye Violet 510. Excitation max: 414 nm, Emission Max: 508 nm

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