Anti-Human p63 (deltaN) Antibody, Clone Poly6190

Rabbit polyclonal antibody against human p63 (deltaN)
Catalog #
60154_C
Rabbit polyclonal antibody against human p63 (deltaN)
From: 540 USD

Overview

The Poly6190 antibody reacts with the deltaN isoforms of human p63, a member of the p53 family of transcription factors. Alternative splicing of the p63 transcript gives rise to two classes of isoforms. The TA isoforms (TA p63-α, -β, -γ) possess an N-terminal transactivation domain, while the deltaN isoforms (deltaN p63-α, -β, -γ) lack the transactivation domain. The TA isoforms are capable of transactivating p53 targets, whereas they antagonize p53. The deltaN isoforms are generally dominant to the TA isoforms and repress the latter. The p63 protein is localized to the nucleus and plays an important role in epithelial stem cell regeneration, epidermal morphogenesis, limb development, and stratification.
Subtype
Primary Antibodies
Target Antigen
p63 (deltaN)
Alternative Names
TP63, Tumor protein P63
Reactive Species
Human
Conjugation
Unconjugated
Host Species
Rabbit
Cell Type
Airway Cells, Dermal Cells
Application
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blotting
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
Clone
Not applicable (poly6190)
Gene ID
8626
Isotype
IgG

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Human p63 (deltaN) Antibody, Clone Poly6190
Catalog #
60154
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human p63 (deltaN) Antibody, Clone Poly6190
Catalog #
60154
Lot #
All
Language
English

Educational Materials (2)

Brochure
Reliable Antibodies for Your Research
Brochure
PneumaCult™ Culture Media for Human Airway Epithelial Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Data and Publications

Data

(A) Primary human airway epithelial cells were expanded in a submerged culture with PneumaCult™-Ex Medium (Catalog #05008) and labeled with Anti- Human p63 (deltaN) Antibody, Clone Poly6190, followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594. (B) DAPI counterstaining of the cells shown in figure (A). (C) Primary human airway epithelial cells cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface were cryo-sectioned and labeled with Anti-Human p63 (deltaN) Antibody, Clone Poly6190 (red), followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594 (red), and antihuman NGF Receptor/p75NTR (CD271) antibody, followed by a donkey anti-mouse IgG antibody, Alexa Fluor® 488 (green). (D) Western blot analysis of non-transfected HEK293 cell lysates (negative control; lane 1) and p63 (deltaN)-transfected HEK293 cell lysates (lane 2). Lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with Anti-Human p63 (deltaN) Antibody, Clone Poly6190. Proteins were visualized using an HRP-conjugated donkey anti-rabbit IgG secondary antibody and chemiluminescence detection.

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