CHO and Other Cell Line Development

Single-cell cloning is usually performed to identify high producing clones exhibiting robust growth characteristics. See MoreThe challenge at this stage is that most cells grow poorly at the low cell densities used for single-cell cloning. Addition of our specially formulated animal component-free supplement supports growth at low cell densities and improves cloning efficiencies by up to 110% compared to DMEM + 10% FBS. For cloning by the limiting dilution method, we offer a chemically defined animal component-free liquid growth medium. Addition of our animal component-free supplement can greatly improve cloning efficiencies at this critical step. For semi-solid cloning methods, ClonaCell™ saves time by combining the selection and cloning steps, as each individual colony is suspended in specialized semi-solid CHO media. To customize the semi-solid medium, use our ClonaCell™ FLEX, a base semi-solid medium to which a growth medium of choice is added.