CHO and Other Cell Line Development

After cell line cloning has been completed, the CHO cell lines expressing the protein of interest are typically expanded and, if desired, slowly adapted to growth in chemically defined animal-component free liquid media for scale-up. See MoreThe expression level of the protein of interest should be monitored for several passages to ensure stable integration of the transgene and robust growth of the cell line. Amplification of the transgene and a concomitant increase in expression of the recombinant protein can be achieved at this stage if a suitable selectable marker such as the DHFR gene has been co-transfected into the cells (see Transfection and Selection above).

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Cell Culture Media and Supplements 4 (3)

Tissue and Cell Culture Dissociation Reagents 4 (1)

Cell Storage Media 4 (3)

Cell Dyes and Detection Assay Kits 4 (8)

Cultureware and General Supplies 4 (1)