CHO and Other Cell Line Development
After cell line cloning has been completed, the CHO cell lines expressing the protein of interest are typically expanded and, if desired, slowly adapted to growth in chemically defined animal-component free liquid media for scale-up.
Products
Cell Culture Media and Supplements 4 (3)
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ClonaCell™-CHO ACF Supplement
Animal component-free, serum-free, glutamine-free 40X supplement for cloning during CHO cell line development -
ClonaCell™-CHO CD Liquid Medium
Chemically defined, animal component-free, serum-free, protein-free, glutamine-free liquid medium for culturing CHO cells -
ClonaCell™-CHO AOF Supplement
Animal origin-free, serum-free, L-glutamine-free, and phenol red-free 40X culture supplement for cloning during CHO cell line development
Tissue and Cell Culture Dissociation Reagents 4 (1)
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Trypsin-EDTA (0.05%)
Enzymatic cell dissociation reagent
Cell Storage Media 4 (3)
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CryoStor® CS5
Animal component-free, defined cryopreservation medium with 5% DMSO -
CryoStor® CSB
Animal component-free and protein-free cryopreservation formulation -
CryoStor® CS10
Animal component-free, defined cryopreservation medium with 10% DMSO
Cell Dyes and Detection Assay Kits 4 (8)
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3% Acetic Acid with Methylene Blue
Reagent for manual counting of nucleated mammalian cells -
Trypan Blue
Reagent for counting viable mammalian cells -
GloCell™ Fixable Viability Dye Violet 540
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye Violet 510
Amine-labeling fluorescent dye for live/dead staining of mammalian cells