Semi-solid methylcellulose-based medium for selecting and cloning suspension-adapted and adherent cells (serum-containing)
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Benefits of semi-solid cloning:
• Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated.
• Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution.
• Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures.
• Pre-selected serum
• Bovine serum albumin
• Phenol red
• Other ingredients
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (6)
Frequently Asked Questions
Is the serum in ClonaCell™-TCS medium heat inactivated?
Is there any IgG in ClonaCell™ TCS?
Can ClonaCell™-TCS be used with any cell line?
Why do I get more cells when I select my fusion in liquid medium rather than in methylcellulose-based semi-solid medium?
How do I thaw ClonaCell™ methylcellulose-based semi-solid medium?
How do I measure and dispense methylcellulose semi-solid medium?
My ClonaCell™ methylcellulose semi-solid medium appears runny. Why does this happen?
What is the optimal number of colonies per plate?
There are still bubbles in the media after I plate my cells. Do I need to disrupt the bubbles?
Do I ever need to re-clone cultures grown with ClonaCell™ semi-solid medium?
Once I pick the colonies and grow the cells in plates, will the residual methylcellulose interfere with characterization? For example, will I have problems doing an ELISA?
How important is the incubator humidity when culturing in methylcellulose-based medium?
Do I have to use 100 mm petri dishes or can I use other cultureware?
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.