G418
Antibiotic for selecting transfected mammalian cells
Overview
Antibiotic G418 is an aminoglycoside related to gentamicin and is toxic to bacteria, yeast, higher plants, mammalian cells, protozoans, and helminths. G418 binds to 80S ribosomes in prokaryotic and eukaryotic cells and inhibits protein synthesis, resulting in cell death. Resistance to the antibiotic is conferred by aminoglycoside-3-phosphotransferase. Vectors containing the G418 resistance gene (neo) are commonly used for transfecting eukaryotic cells, with selection of transfected cells achieved by culturing them in the presence of G418. Cells may exhibit variable resistance to the antibiotic and can take up to a week to die (adherent cells may be more sensitive).
The G418 antibiotic is suitable for use in cell culture for the selection and maintenance of G418-resistant clones when using ClonaCell™-TCS Medium (Catalog #03814), ClonaCell™-CHO CD Medium (Catalog #03815), ClonaCell™-CHO ACF Medium (Catalog #03816), ClonaCell™CHO CD Liquid Medium (Catalog #03817), or other cell culture media.
The G418 antibiotic is suitable for use in cell culture for the selection and maintenance of G418-resistant clones when using ClonaCell™-TCS Medium (Catalog #03814), ClonaCell™-CHO CD Medium (Catalog #03815), ClonaCell™-CHO ACF Medium (Catalog #03816), ClonaCell™CHO CD Liquid Medium (Catalog #03817), or other cell culture media.
Cell Type
CHO Cells, Hybridomas
Species
Mouse
Application
Hybridoma Generation, Toxicity Assay
Area of Interest
Cell Line Development, Drug Discovery and Toxicity Testing, Hybridoma Generation
CAS Number
108321-42-2
Related Products
Related Products
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
G418
|
Catalog # 03812 |
Lot # All |
Language English |
|
Document Type
Safety Data Sheet
|
Product Name
G418
|
Catalog # 03812 |
Lot # All |
Language English |
Educational Materials(2)
Brochure
Standardized Solutions for Mouse Pluripotent Stem Cells
Brochure
ClonaCell™ Media for Hybridoma and Cell Line Development
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
