ClonaCell™-CHO CD Medium
Chemically defined, animal component-free, serum-free, protein-free, glutamine-free semi-solid methylcellulose-based medium for selecting and cloning CHO cells
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Benefits of semi-solid cloning:
• Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct monoclonal colonies. This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening.
• Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning.
• Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion.
• Kolliphor® P188
• Other ingredients
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (7)
Frequently Asked Questions
Are there any selective agents in ClonaCell™-CHO ACF or ClonaCell™-CHO CD?
My cells grow fine in ClonaCell™-CHO ACF but I do not see colony growth in ClonaCell™-CHO CD. Why?
What medium do you recommend for the expansion of clones after selection in ClonaCell™-CHO ACF or ClonaCell™-CHO CD?
How many cells should I plate for cloning in ClonaCell™-CHO CD?
Is the serum in ClonaCell™-TCS medium heat inactivated?
Is there any IgG in ClonaCell™ TCS?
Can ClonaCell™-TCS be used with any cell line?
Why do I get more cells when I select my fusion in liquid medium rather than in methylcellulose-based semi-solid medium?
How do I thaw ClonaCell™ methylcellulose-based semi-solid medium?
How do I measure and dispense methylcellulose semi-solid medium?
My ClonaCell™ methylcellulose semi-solid medium appears runny. Why does this happen?
What is the optimal number of colonies per plate?
There are still bubbles in the media after I plate my cells. Do I need to disrupt the bubbles?
Do I ever need to re-clone cultures grown with ClonaCell™ semi-solid medium?
Once I pick the colonies and grow the cells in plates, will the residual methylcellulose interfere with characterization? For example, will I have problems doing an ELISA?
How important is the incubator humidity when culturing in methylcellulose-based medium?
Do I have to use 100 mm petri dishes or can I use other cultureware?
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ClonaCell™-CHO CD Medium
Kolliphor® is a registered trademark of BASF.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.