Hybridoma Production

After selection and cloning of hybridomas has been performed, clones that test positive for antibody production are propagated in a specialized rich growth medium to promote expansion and to wean the hybridomas off the aminopterin used during selection. See MoreHybridomas are then typically adapted and maintained in a suitable routine growth medium . Cryopreservation of the cells in a viable form can be achieved by freezing them in a cryopreservation medium and storing them in liquid nitrogen to secure the supply of the hybridoma.