Hygromycin B

Antibiotic for selecting transfected prokaryotic and eukaryotic cells
Hygromycin B

Antibiotic for selecting transfected prokaryotic and eukaryotic cells

100 mg
Catalog # 03813
138 USD

Overview

Hygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome subunit and inducing misreading of the mRNA template. Hygromycin B has been used to select transfectants in a wide variety of cells including bacteria, protozoans, yeast, fungi, plants, and mammalian cells. Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it. Hygromycin B is an effective agent for the selection and maintenance of bacterial and eukaryotic cells stably transfected with vectors containing E. coli Hygromycin B resistance gene (hyg or hph).

This antibiotic is suitable for use in cell culture for the selection of hygromycin-resistant clones when using ClonaCell™-TCS Medium (Catalog #03814), and for the selection of any hygromycin-resistant transfected cell type.
Subtype
Supplements
Cell Type
CHO Cells, Hybridomas
Species
Mouse
Application
Cell Culture, Hybridoma Generation
Area of Interest
Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing, Hybridoma Generation

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Hygromycin B
Catalog #
03813
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Hygromycin B
Catalog #
03813
Lot #
All
Language
English

Educational Materials(2)

Brochure
Standardized Solutions for Mouse Pluripotent Stem Cells
Brochure
ClonaCell™ Media for Hybridoma and Cell Line Development

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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