Intestinal Epithelial Cell Research

Intestinal stem cells (ISCs) are located in the base of the intestinal crypt and give rise to all of the intestinal epithelial cell types including Paneth cells, goblet cells, enteroendocrine cells and enterocytes. See MoreThe rapid proliferation and differentiation of ISCs allows the intestinal epithelium to regenerate itself every four to five days and make the tissue an attractive subject for a variety of research applications. Harvest ISCs by isolating intestinal crypts from primary tissue using our non-enzymatic cell dissociation reagent. . To generate convenient and physiological models of the intestine, use the intestinal crypts to establish intestinal organoid cultures. .

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Technical Bulletin Intestinal Epithelial Organoid Culture with IntestiCult™ Organoid Growth Medium (Mouse)
Video (11:17) Mouse Intestinal Organoid Culture with IntestiCult™ Organoid Growth Medium
Mini Review Intestinal Organoid Culture
Webinar (1:03:40) From Stem Cells to Organoids: Modeling the Gastrointestinal Tract
Video (6:17) Mouse Intestinal Organoid Passaging with IntestiCult™ Organoid Growth Medium

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Products

Cell Culture Media and Supplements 4 (1)

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DMEM/F-12 with 15 mM HEPES

Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

Tissue and Cell Culture Dissociation Reagents 4 (3)

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Gentle Cell Dissociation Reagent

cGMP, enzyme-free cell dissociation reagent
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Collagenase A, ACF

Animal component-free collagenase for the digestion of native collagen fibrils
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Collagenase Type I

For digestion of native collagen fibrils

Buffers and Solutions 4 (1)

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D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

Cell Dyes and Detection Assay Kits 4 (2)

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DAPI (Hydrochloride)

DNA-labeling dye
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Trypan Blue

Reagent for counting viable mammalian cells

Primary and Cultured Cells 4 (1)

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Mouse Intestinal Organoids

Cryopreserved mouse intestinal epithelial organoids for establishment of organoid cultures

Cultureware and General Supplies 4 (1)

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Reversible Strainers

Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids

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