Intestinal Epithelial Organoid Culture with IntestiCult™ Organoid Growth Medium (Mouse)


  • Document # 28223
  • Version 2.0.1
  • Dec 2016

3D Culture Systems

The use of three-dimensional (3D) culture systems continues to increase, due in large part to their potential to facilitate basic research into multicellular biological mechanisms as well as bridge the gap between traditional model systems such as immortalized cell lines or animal models and true human physiology.1,2 Adoption of 3D culture systems has been driven by growing recognition that organotypic cultures can provide a more physiologically relevant in vitro model than traditional monolayer culture techniques.1,2

Intestinal Organoids

Mouse intestinal organoid

Figure 1. Light Microscope Visualization (10X) of a Mouse Intestinal Epithelial Organoid After Five Days of Culture

In the intestinal epithelial organoid culture system, intestinal stem cells recapitulate both the self-renewal capacity and differentiation hierarchy observed in the adult intestine in vivo (Figure 1). In addition to exhibiting the cell types present in the adult intestinal epithelium, intestinal organoids are characterized by a crypt-villus organization, epithelial polarization, and functional lumen,3,4 which together make this culture system a powerful tool for investigating intestinal biology and the properties of intestinal stem cells.3,4 Potential contributions to regenerative medicine are also being explored, as organoids have been demonstrated to incorporate into the colon of chemically injured mice to form segments that are morphologically indistinguishable from the surrounding epithelium.5 Intestinal organoids have been successfully co-cultured with both bacterial and immune cells, providing a model to study cell-to-cell interactions between epithelial tissues and the immune system.6,7 As well, mouse intestinal organoids are amenable to genetic manipulations using the CRISPR-Cas system and infection with retroviruses and lentiviruses.8,9

Protocols for Mouse Intestinal Organoid Culture

Find a series of step-by-step procedures and videos for the isolation, culture, passaging, and cryopreservation of mouse small intestinal and colonic crypts using the IntestiCult™ Organoid Growth Medium (Mouse).

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Read additional considerations and technical tips for culturing mouse intestinal organoids >

Tools and Technologies

Intestinal Organoid

IntestiCult™ Intestinal Organoid Culture Media

Establish, expand, maintain, and further differentiate intestinal organoid cultures from human or mouse intestinal crypts using IntestiCult™ complete and defined growth media.

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Try IntestiCult™ Organoid Growth Medium (Mouse) in your own lab for efficient establishment and long-term maintenance of mouse intestinal organoids from intestinal crypts or single intestinal stem cells.

Training Programs

Intestinal Organoid Course

On-Demand Intestinal Organoid Course

Access free, self-paced training on how to culture mouse intestinal organoids.

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Intestinal Organoid Culture

Virtual or In-Person Intestinal Organoid Training

Gain experience in growing mouse intestinal organoids, with guidance from our team of scientific experts.

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