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IntestiCult™ Organoid Growth Medium (Mouse)

Cell culture medium for establishment and maintenance of mouse intestinal organoids

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Interested in trying STEMCELL’s organoid products for your intestinal research? Fill out the form to request information about introductory offers.

IntestiCult™ Organoid Growth Medium (Mouse)

Cell culture medium for establishment and maintenance of mouse intestinal organoids

From: 384 USD
Catalog #
06005_C
Cell culture medium for establishment and maintenance of mouse intestinal organoids
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Product Advantages


  • Convenient, in vitro system that recapitulates the identity and organization of the adult intestinal epithelium, including intra- and intercellular signaling, self-propagating stem cell niche and functional transport into and out of the lumen

  • Serum-free and defined medium formulation that delivers consistent results

  • Enables generation of intestinal organoids in less than one week

  • Simple format and easy-to-use protocol

What's Included

  • IntestiCult™ OGM Mouse Basal Medium, 90 mL
  • IntestiCult™ OGM Mouse Supplement 1, 5 mL
  • IntestiCult™ OGM Mouse Supplement 2, 5 mL
Interested in trying STEMCELL’s organoid products for your intestinal research? Fill out the form to request information about introductory offers.
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. DMEM/F-12 with 15 mM HEPES
    DMEM/F-12 with 15 mM HEPES

    Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

  3. D-PBS (Without Ca++ and Mg++)
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

IntestiCult™ Organoid Growth Medium (Mouse) is a defined, serum-free cell culture medium for efficient establishment and long-term maintenance of mouse intestinal organoids.These organoids, or “mini-guts”, provide a convenient in vitro organotypic culture system for studying both the small and large intestinal epithelium and associated stem cell dynamics. Organoids grown in IntestiCult™ feature a polarized epithelium that contains all of the known cell types of the adult intestinal epithelium. Individual intestinal crypts rapidly form organoids when cultured in IntestiCult™ Organoid Growth Medium (Mouse). Applications of these cultures include studying the development and function of the normal and tumorigenic intestinal epithelium, modeling intestinal disease, and investigating stem cell properties and regenerative therapy approaches. Organoid culture enables convenient in vitro characterization of a system with strong physiological relevance to the adult intestine.

Should you intend to use this product for commercial purposes, please contact HUB at www.huborganoids.nl for a commercial use license or for clarifications in relation to HUB licensing.
Subtype
Specialized Media
Cell Type
Intestinal Cells
Species
Mouse
Application
Cell Culture, Differentiation, Expansion, Maintenance, Organoid Culture
Brand
IntestiCult
Area of Interest
Cancer, Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Stem Cell Biology
Formulation Category
Serum-Free

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
IntestiCult™ Organoid Growth Medium (Mouse)
Catalog #
06005
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
IntestiCult™ Organoid Growth Medium (Mouse)
Catalog #
06005
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
IntestiCult™ Organoid Growth Medium (Mouse)
Catalog #
06005
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
IntestiCult™ Organoid Growth Medium (Mouse)
Catalog #
06005
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
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Resources and Publications

Educational Materials (39)

Intestinal Organoids
Brochure
Intestinal Organoids
Organoids
Brochure
Organoids
Intestinal Epithelial Organoid Culture with IntestiCult™ Organoid Growth Medium (Mouse)
Technical Bulletin
Intestinal Epithelial Organoid Culture with IntestiCult™ Organoid Growth Medium (Mouse)
How to Generate Mouse Intestinal Organoid-Derived Monolayers Using IntestiCult™
Protocol
How to Generate Mouse Intestinal Organoid-Derived Monolayers Using IntestiCult™
How to Passage Mouse Intestinal Organoids
Protocol
How to Passage Mouse Intestinal Organoids
Performing Immunocytochemical Staining of Epithelial Organoids
Protocol
Performing Immunocytochemical Staining of Epithelial Organoids
How to Cryopreserve Intestinal Organoids
Protocol
How to Cryopreserve Intestinal Organoids
How to Isolate Mouse Intestinal Crypts
Protocol
How to Isolate Mouse Intestinal Crypts
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
Protocol
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
How to Thaw Intestinal Organoids
Protocol
How to Thaw Intestinal Organoids
How to Establish Intestinal Organoid Culture from Isolated Mouse Intestinal Crypts
Protocol
How to Establish Intestinal Organoid Culture from Isolated Mouse Intestinal Crypts
SnapShot: Growing Organoids from Stem Cells
Wallchart
SnapShot: Growing Organoids from Stem Cells
Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope
1:07
Video
Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope
Intestinal Organoid Culture: The Next Dimension in Intestinal Research
1:50
Video
Intestinal Organoid Culture: The Next Dimension in Intestinal Research
How to Count Intestinal Crypts for Mouse Organoid Cultures
1:09
Video
How to Count Intestinal Crypts for Mouse Organoid Cultures
How to Culture Mouse Intestinal Organoids: Isolating Intestinal Crypts and Establishing Organoids
11:17
Video
How to Culture Mouse Intestinal Organoids: Isolating Intestinal Crypts and Establishing Organoids
Why Culture Intestinal Organoids with IntestiCult™ Organoid Growth Medium
3:19
Video
Why Culture Intestinal Organoids with IntestiCult™ Organoid Growth Medium
How to Culture Mouse Intestinal Organoids: Passaging
6:17
Video
How to Culture Mouse Intestinal Organoids: Passaging
How to Culture Mouse Intestinal Organoids: Introduction
1:09
Video
How to Culture Mouse Intestinal Organoids: Introduction
Challenges in Translating iPSC Technology
41:47
Webinar
Challenges in Translating iPSC Technology
Organoid Expert Panel
52:35
Webinar
Organoid Expert Panel
Nature Research Round Table: The Promise of Organoid Medicine
17:17
Webinar
Nature Research Round Table: The Promise of Organoid Medicine
Nature Research Round Table: Self-Organization and Symmetry Breaking in Intestinal Organoid Development
11:52
Webinar
Nature Research Round Table: Self-Organization and Symmetry Breaking in Intestinal Organoid Developm...
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
24:56
Webinar
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
Spotlight on Organoids: Expert Panel
Webinar
Spotlight on Organoids: Expert Panel
From Stem Cells to Organoids: Modeling the Gastrointestinal Tract
1:03:40
Webinar
From Stem Cells to Organoids: Modeling the Gastrointestinal Tract
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
17:20
Webinar
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
Nature Research Round Table: Organoid Modeling of Stem Cells and Disease Microenvironments
4:47
Webinar
Nature Research Round Table: Organoid Modeling of Stem Cells and Disease Microenvironments
Nature Research Round Table: Organoids as Models for Human Disease
15:10
Webinar
Nature Research Round Table: Organoids as Models for Human Disease
Modeling Host-Microbe Interactions Using Human Intestinal Organoids
16:31
Webinar
Modeling Host-Microbe Interactions Using Human Intestinal Organoids
Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
56:31
Webinar
Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
1:10:09
Webinar
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
Nature Research Round Table: Modeling Congenital Pediatric Diarrhea in Intestinal Enteroids
15:15
Webinar
Nature Research Round Table: Modeling Congenital Pediatric Diarrhea in Intestinal Enteroids
Nature Research Round Table: Modeling Intestinal Development In Vivo and In Vitro
10:17
Webinar
Nature Research Round Table: Modeling Intestinal Development In Vivo and In Vitro
Intestinal Organoid Culture
Mini Review
Intestinal Organoid Culture
Highly Efficient Differentiation of Human Pluripotent Stem Cells into Long-term Expandable “Mini-gut” Organoids
Scientific Poster
Highly Efficient Differentiation of Human Pluripotent Stem Cells into Long-term Expandable “Mini-g...
Efficient Establishment and Long-Term Maintenance of 3-Dimensional Mouse Intestinal Organoids Using a Novel Defined and Serum-Free Medium
Scientific Poster
Efficient Establishment and Long-Term Maintenance of 3-Dimensional Mouse Intestinal Organoids Using ...
Development of a 96-well Assay for Assessing Cell Viability in Mouse Small Intestinal-Derived Organoids after Treatment with Cytotoxic Compounds
Scientific Poster
Development of a 96-well Assay for Assessing Cell Viability in Mouse Small Intestinal-Derived Organo...
Heather McCauley, PhD
Interview
Heather McCauley, PhD

Publications (95)

CRL3-SPOP ubiquitin ligase complex suppresses the growth of diffuse large B-cell lymphoma by negatively regulating the MyD88/NF-$\kappa$B signaling. X. Jin et al. Leukemia 2020 may

Abstract

Recurrent oncogenic mutations of MyD88 have been identified in a variety of lymphoid malignancies. Gain-of-function mutations of MyD88 constitutively activate downstream NF-$\kappa$B signaling pathways, resulting in increased cellular proliferation and survival. However, whether MyD88 activity can be aberrantly regulated in MyD88-wild-type lymphoid malignancies remains poorly understood. SPOP is an adaptor protein of CUL3-based E3 ubiquitin ligase complex and frequently mutated genes in prostate and endometrial cancers. In this study, we reveal that SPOP binds to and induces the nondegradative ubiquitination of MyD88 by recognizing an atypical SPOP-binding motif in MyD88. This modification blocks Myddosome assembly and downstream NF-$\kappa$B activation. SPOP is mutated in a subset of lymphoid malignancies, including diffuse large B-cell lymphoma (DLBCL). Lymphoid malignancies-associated SPOP mutants exhibited impaired binding to MyD88 and suppression of NF-$\kappa$B activation. The DLBCL-associated, SPOP-binding defective mutants of MyD88 escaped from SPOP-mediated ubiquitination, and their effect on NF-$\kappa$B activation is stronger than that of wild-type MyD88. Moreover, SPOP suppresses DLBCL cell growth in vitro and tumor xenograft in vivo by inhibiting the MyD88/NF-$\kappa$B signaling. Therefore, SPOP acts as a tumor suppressor in DLBCL. Mutations in the SPOP-MyD88 binding interface may disrupt the SPOP-MyD88 regulatory axis and promote aberrant MyD88/NF-$\kappa$B activation and cell growth in DLCBL.
View publication
Hormonal Suppression of Stem Cells Inhibits Symmetric Cell Division and Gastric Tumorigenesis. W. Chang et al. Cell stem cell 2020 may

Abstract

Cancer is believed to arise from stem cells, but mechanisms that limit the acquisition of mutations and tumor development have not been well defined. We show that a +4 stem cell (SC) in the gastric antrum, marked by expression of Cck2r (a GPCR) and Delta-like ligand 1 (DLL1), is a label-retaining cell that undergoes predominant asymmetric cell division. This +4 antral SC is Notch1low/ Numb+ and repressed by signaling from gastrin-expressing endocrine (G) cells. Chemical carcinogenesis of the stomach is associated with loss of G cells, increased symmetric stem cell division, glandular fission, and more rapid stem cell lineage tracing, a process that can be suppressed by exogenous gastrin treatment. This hormonal suppression is associated with a marked reduction in gastric cancer mutational load, as revealed by exomic sequencing. Taken together, our results show that gastric tumorigenesis is associated with increased symmetric cell division that facilitates mutation and is suppressed by GPCR signaling.
View publication
Iroquois Homeobox Protein 2 Identified as a Potential Biomarker for Parkinson's Disease. H. Sim et al. International journal of molecular sciences 2020 may

Abstract

The diagnosis of Parkinson's disease (PD) is initiated after the occurrence of motor symptoms, such as resting tremors, rigidity, and bradykinesia. According to previous reports, non-motor symptoms, notably gastrointestinal dysfunction, could potentially be early biomarkers in PD patients as such symptoms occur earlier than motor symptoms. However, connecting PD to the intestine is methodologically challenging. Thus, we generated in vitro human intestinal organoids from PD patients and ex vivo mouse small intestinal organoids from aged transgenic mice. Both intestinal organoids (IOs) contained the human LRRK2 G2019S mutation, which is the most frequent genetic cause of familial and sporadic PD. By conducting comprehensive genomic comparisons with these two types of IOs, we determined that a particular gene, namely, Iroquois homeobox protein 2 (IRX2), showed PD-related expression patterns not only in human pluripotent stem cell (PSC)-derived neuroectodermal spheres but also in human PSC-derived neuronal cells containing dopaminergic neurons. We expected that our approach of using various cell types presented a novel technical method for studying the effects of multi-organs in PD pathophysiology as well as for the development of diagnostic markers for PD.
View publication
View All Publications

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Legal Statement:

This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use this product for growing organoids for drug screening aiming for commercial gain or for other commercial purposes. Purchasers wishing to use the product for purposes other than research use should contact HUB to obtain a further license (an “Organoid-Growth License”). Purchasers may apply for an Organoid-Growth License from HUB and such license will not be unreasonably withheld by HUB.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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