Intestinal Organoid Training

Online and in-person training courses to support the culture of intestinal organoids

Intestinal Organoid Virtual Training
Step-by-Step Procedural Lab Videos
Step-by-Step Procedural Lab Videos
Intestinal Organoid Starter Kit

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Intestinal Organoid Virtual Training Starter Kit

Starter Kit to complement virtual training course. Access the Intestinal Organoid Classroom through the link in the blue box below

Registration/Cancellation Deadline 01/01/2019

Catalog #00261

788 USD

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Intestinal Organoid In-Person Training

Next session dates will be based on participant demand and availability

Catalog #00262

1,499 USD

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Already have the Intestinal Organoid Starter Kit? Access the Online Intestinal Organoid Classroom here.

Overview Speakers Agenda Scientific Resources Related Products

Course Overview

Virtual Training (Catalog #00261)

Learn to culture mouse intestinal organoids in a defined and serum-free medium from the convenience of your own lab. Gain access to lectures and step-by-step procedural videos where our scientists demonstrate the entire protocol for growing mouse intestinal organoids.

Participants Receive

1. Intestinal Organoid Starter Kit containing:

- Mouse Intestinal Organoids (Catalog #70931)
- IntestiCult™ Organoid Growth Medium (Mouse) (Catalog #06005)
- Gentle Cell Dissociation Reagent (Catalog #07174)

2. Access online videos in the Intestinal Organoid Classroom through the link in the blue box above.

In-Person Training (Catalog #00262)

This course provides a comprehensive overview of the mouse intestinal organoid culture workflow. Learn to establish cultures from mouse small intestine and colon and how to maintain them in IntestiCult™ Organoid Growth Medium (Mouse). Participants will also gain experience with performing downstream assays.

Participants Receive

Course booklet and electronic versions of instructional material
Certificate following successful completion of the course
Lunch and refreshments on each day and dinner hosted on one of the evenings

Type:

Training and Education

Subtype:

Training Programs

Area of Interest:

Stem Cell Biology; Drug Discovery and Toxicity Testing; Epithelial Cell Biology

Cell Type:

Intestinal Cells

Species:

Mouse

Venues:

Online (Catalog #00261)

Canada:
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6

United Kingdom:
STEMCELL Technologies UK Ltd.
Building 7100, Cambridge Research Park,
Beach Drive, Waterbeach
Cambridge, UK, CB25 9TL

Speakers

Speakers will vary depending on location.

Ryan Conder, PhD

Senior Scientist and Team Lead of the Intestinal Group
STEMCELL Technologies

Salvatore Simmini, PhD

Scientist, Intestinal
STEMCELL Technologies

Course Agenda (Tentative)

Virtual Training (Catalog #00261)

Lecture 1: Introduction to Intestinal Organoid Culture
Lecture 2: Functional Applications of Intestinal Organoid Culture
Session 1:
- Thaw cryopreserved intestinal organoids and culture in IntestiCult™ Organoid Growth Medium (Mouse)
- Observe organoid morphology in culture after 3 hours, 1 day and 2 days
Session 2: Observe time course of organoid morphology in culture and discuss long-term maintenance
Session 3:
- Evaluate optimal organoid morphology for passaging and cryopreservation.
- Passage mouse intestinal organoids and discuss cryopreservation
Session 4: Observe organoid morphology one day after passaging

In-Person Training (Catalog #00262)

Day One

Welcome Presentation and Introductions
Lecture: Overview of Intestinal Organoid Culture
Practical Session: Mouse Intestinal Organoid Morphology Cultured in IntestiCult™ Organoid Growth Medium.
- Observe time course of organoid morphology
- Demonstration and hands-on practice with passaging mouse intestinal organoids
Practical Session: Mouse Crypt Isolation and Establishment of Organoid Culture from Small Intestine and Colon
Wrap Up and Q&A

Day Two

Lecture: Applications of Mouse Intestinal Organoids and Current Development
Practical Session: Mouse Intestinal Organoid Maintenance
- Observe organoid cultures from day 1
- Demonstration of cryopreservation and thawing of mouse intestinal organoids
Practical Session: Downstream Assays for Intestinal Organoids
Wrap Up:
- Q&A
- Quiz
- Course Evaluation
- Certificate Presentation