Intestinal Organoid Training
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Gentle Cell Dissociation ReagentcGMP, enzyme-free cell dissociation reagent
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DMEM/F-12 with 15 mM HEPESDulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
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D-PBS (Without Ca++ and Mg++)Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
Virtual Training (Catalog #00261)
Learn to culture mouse intestinal organoids in a defined and serum-free medium from the convenience of your own lab. Gain access to lectures and step-by-step procedural videos where our scientists demonstrate the entire protocol for growing mouse intestinal organoids.
Participants Receive
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- Mouse Intestinal Organoids (Catalog #70931)
- IntestiCult™ Organoid Growth Medium (Mouse) (Catalog #06005)
- Gentle Cell Dissociation Reagent (Catalog #07174)
Note: Additional materials and reagents are required to grow mouse intestinal organoids. Please see Product Information Sheet.
In-Person Training (Catalog #00262)
This course provides a comprehensive overview of the mouse intestinal organoid culture workflow. Learn to establish cultures from mouse small intestine and colon and how to maintain them in IntestiCult™ Organoid Growth Medium (Mouse). Participants will also gain experience with performing downstream assays.
Participants Receive
- Course booklet and electronic versions of instructional material
- Certificate following successful completion of the course
- Lunch and refreshments on each day and dinner hosted on one of the evenings
Canada:
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6
United Kingdom:
STEMCELL Technologies UK Ltd.
Building 7100, Cambridge Research Park,
Beach Drive, Waterbeach
Cambridge, UK, CB25 9TL
Speakers
Speakers will vary depending on location.
STEMCELL Technologies
STEMCELL Technologies
Agenda
Virtual Training (Catalog #00261)
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Lecture 1: Introduction to Intestinal Organoid Culture
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Lecture 2: Functional Applications of Intestinal Organoid Culture
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Session 1:
- Thaw cryopreserved intestinal organoids and culture in IntestiCult™ Organoid Growth Medium (Mouse)
- Observe organoid morphology in culture after 3 hours, 1 day and 2 days
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Session 2: Observe time course of organoid morphology in culture and discuss long-term maintenance
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Session 3:
- Evaluate optimal organoid morphology for passaging and cryopreservation.
- Passage mouse intestinal organoids and discuss cryopreservation
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Session 4: Observe organoid morphology one day after passaging
In-Person Training (Catalog #00262)
Day One
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Welcome Presentation and Introductions
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Lecture: Overview of Intestinal Organoid Culture
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Practical Session: Mouse Intestinal Organoid Morphology Cultured in IntestiCult™ Organoid Growth Medium.
- Observe time course of organoid morphology
- Demonstration and hands-on practice with passaging mouse intestinal organoids
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Practical Session: Mouse Crypt Isolation and Establishment of Organoid Culture from Small Intestine and Colon
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Wrap Up and Q&A
Day Two
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Lecture: Applications of Mouse Intestinal Organoids and Current Development
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Practical Session: Mouse Intestinal Organoid Maintenance
- Observe organoid cultures from day 1
- Demonstration of cryopreservation and thawing of mouse intestinal organoids
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Practical Session: Downstream Assays for Intestinal Organoids
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Wrap Up:
- Q&A
- Quiz
- Course Evaluation
- Certificate Presentation
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet 1
|
Product Name
Intestinal Organoid Virtual Training Starter Kit
|
Catalog #
00261 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet 2
|
Product Name
Intestinal Organoid Virtual Training Starter Kit
|
Catalog #
00261 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet 1
|
Product Name
Intestinal Organoid In-Person Training
|
Catalog #
00262 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet 2
|
Product Name
Intestinal Organoid In-Person Training
|
Catalog #
00262 |
Lot #
All |
Language
English |
Educational Materials (5)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Quality Statement:
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
