Intestinal Organoid Training
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Intestinal Organoid Virtual Training -
Step-by-Step Procedural Lab Videos -
Step-by-Step Procedural Lab Videos -
Intestinal Organoid Starter Kit
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Course Overview
Virtual Training (Catalog #00261)
Learn to culture mouse intestinal organoids in a defined and serum-free medium from the convenience of your own lab. Gain access to lectures and step-by-step procedural videos where our scientists demonstrate the entire protocol for growing mouse intestinal organoids. Registration includes an Intestinal Organoid Starter Kit containing cryopreserved mouse intestinal organoids and reagents to grow your own organoids.
Participants Receive
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- Mouse Intestinal Organoids (Catalog #70931)
- IntestiCult™ Organoid Growth Medium (Mouse) (Catalog #06005)
- Gentle Cell Dissociation Reagent (Catalog #07174)
Note: Additional materials and reagents are required to grow mouse intestinal organoids. Please see Product Information Sheet.
In-Person Training (Catalog #00262)
This course provides a comprehensive overview of the mouse intestinal organoid culture workflow. Learn to establish cultures from mouse small intestine and colon and how to maintain them in IntestiCult™ Organoid Growth Medium (Mouse). Participants will also gain experience with performing downstream assays.
Participants Receive
- Course booklet and electronic versions of instructional material
- Certificate following successful completion of the course
- Lunch and refreshments on each day and dinner hosted on one of the evenings
Canada:
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6
United Kingdom:
STEMCELL Technologies UK Ltd.
Building 7100, Cambridge Research Park,
Beach Drive, Waterbeach
Cambridge, UK, CB25 9TL
Speakers
Speakers will vary depending on location.
STEMCELL Technologies
STEMCELL Technologies
Course Agenda (Tentative)
Virtual Training (Catalog #00261)
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Lecture 1: Introduction to Intestinal Organoid Culture
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Lecture 2: Functional Applications of Intestinal Organoid Culture
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Session 1:
- Thaw cryopreserved intestinal organoids and culture in IntestiCult™ Organoid Growth Medium (Mouse)
- Observe organoid morphology in culture after 3 hours, 1 day and 2 days
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Session 2: Observe time course of organoid morphology in culture and discuss long-term maintenance
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Session 3:
- Evaluate optimal organoid morphology for passaging and cryopreservation.
- Passage mouse intestinal organoids and discuss cryopreservation
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Session 4: Observe organoid morphology one day after passaging
In-Person Training (Catalog #00262)
Day One
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Welcome Presentation and Introductions
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Lecture: Overview of Intestinal Organoid Culture
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Practical Session: Mouse Intestinal Organoid Morphology Cultured in IntestiCult™ Organoid Growth Medium.
- Observe time course of organoid morphology
- Demonstration and hands-on practice with passaging mouse intestinal organoids
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Practical Session: Mouse Crypt Isolation and Establishment of Organoid Culture from Small Intestine and Colon
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Wrap Up and Q&A
Day Two
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Lecture: Applications of Mouse Intestinal Organoids and Current Development
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Practical Session: Mouse Intestinal Organoid Maintenance
- Observe organoid cultures from day 1
- Demonstration of cryopreservation and thawing of mouse intestinal organoids
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Practical Session: Downstream Assays for Intestinal Organoids
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Wrap Up:
- Q&A
- Quiz
- Course Evaluation
- Certificate Presentation
Scientific Resources
Product Documentation
Educational Materials
(5)
Related Products
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IntestiCult™ Organoid Growth Medium (Mouse)
Cell culture medium for establishment and maintenance of mouse intestinal organoids -
IntestiCult™ Organoid Growth Medium (Human)
Cell culture medium for establishment and maintenance of human intestinal organoids -
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Animal component-free, defined cryopreservation medium with 10% DMSO