Intestinal Organoid Training

Online and in-person training courses to support the culture of intestinal organoids

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Intestinal Organoid Virtual Training

Kits will be shipped during the week of September 25-29, 2017
Course will be held on October 17-25, 2017

Registration/Cancellation Deadline 09/15/2017
Catalog #00261
750 USD

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Course Overview

Virtual Training (00261) Details

October 17 - 25, 2017 (Four Sessions with 10am CST Start Times)

Stem Cell Biology; Drug Discovery and Toxicity Testing; Epithelial Cell Biology
Intestinal Cells
Mouse
One Week - Four Sessions
Online

Learn to culture mouse intestinal organoids in a defined and serum-free medium from the convenience of your own lab. Over the span of four sessions, interact with our scientists as they demonstrate the entire protocol through streaming procedural videos and answer your questions during live Q&A sessions. Registration includes an Intestinal Organoid Starter Kit containing cryopreserved mouse intestinal organoids and reagents to establish your own organoids in parallel with our online instructors.

Participants Receive

  • Session 1: Thaw cryopreserved intestinal organoids and culture in IntestiCult™ Organoid Growth Medium (Mouse). Observe organoid morphology in culture after 3 hours, 1 day and 2 days
  • Session 2: Observe time course of organoid morphology in culture and discuss long-term maintenance
  • Session 3: Evaluate optimal organoid morphology for passaging and cryopreservation. Passage mouse intestinal organoids and discuss cryopreservation
  • Session 4: Observe organoid morphology one day after passaging
  • Lecture topics include:
  • Introduction to intestinal research and organoid culture techniques
  • Procedure to grow organoids from cryopreserved mouse intestinal organoids using IntestiCult™ Organoid Growth Medium (Mouse)

Speakers

 

Dr. Ryan Conder
Dr. Ryan Conder
Senior Scientist and Team Lead of the Intestinal Group
STEMCELL Technologies

Dr. Ryan Conder is a Senior Scientist and Team Lead of the Intestinal Group in the Research and Development Department at STEMCELL Technologies. His group is responsible for products relating to 3D intestinal organoid culture, including IntestiCult™ Organoid Growth Medium (Mouse). Prior to Joining STEMCELL Technologies in 2012, Dr. Conder investigated the mechanisms regulating proliferation and differentiation of adult intestinal stem cells in the group of Dr. Juergen Knoblich at the Institute for Molecular Biotechnology of the Austrian Academy of Sciences. He is also a principle investigator on the Horizon 2020 European Research Council INTENS project.

Course Agenda (Tentative)

Virtual Training

  • Week of September 25, 2017
    Intestinal Organoid Starter Kits will be shipped.
    Participants to receive kits within 1 week.
  • Session 1: Tuesday, October 17, 2017 (10:00 am -11:30 am CST)
    Welcome and Introductions
    Lecture: Introduction to Intestinal Organoid Culture
    Protocol Videos:
    • Thaw cryopreserved intestinal organoids and culture in IntestiCult™ Organoid Growth Medium (Mouse)
    • Observe organoid morphology in culture after 3 hours, 1 day and 2 days
    • Q&A
  • Session 2: Thursday, October 19, 2017 (10:00 am - 11:00 am CST)
    Recap and Q&A
    Protocol Videos:
    • Observe time course of organoid morphology in culture
    • Discuss long-term culture maintenance in IntestiCult™ Organoid Growth Medium (Mouse)
    • Q&A
  • Session 3: Monday, October 23, 2017 (10:00 am - 11:00 am CST)
    Recap and Q&A
    Protocol Videos:
    • Evaluate optimal organoid morphology for passaging and cryopreservation
    • Passage mouse intestinal organoids and discuss cryopreservation in CryoStor® CS10
    • Review submitted organoid images and Q&A
  • Session 4: Wednesday, October 25, 2017 (10:00 am - 11:00 am CST)
    Recap and Q&A
    Protocol Videos:
    • Observe organoid morphology one day after passaging
    • Q&A

Policy

Virtual Training (00261)

Cancellation

A 90% refund will be issued if notification is received in writing more than 30 days in advance of the course date. No refund will be given with less than 30 days’ notice unless we are able to find another participant to fill the position.

STEMCELL Technologies reserves the right to cancel the course if we do not receive a minimum number of registrations 30 days in advance of the course date. Should the course be cancelled, your payment will be refunded in full.

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