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IntestiCult™-SF Organoid Growth Medium (Human)

Serum-free and conditioned medium-free cell culture medium for establishment and maintenance of human intestinal organoids

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IntestiCult™-SF Organoid Growth Medium (Human)

Serum-free and conditioned medium-free cell culture medium for establishment and maintenance of human intestinal organoids

From: 648 USD
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Serum-free and conditioned medium-free cell culture medium for establishment and maintenance of human intestinal organoids
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Product Advantages


  • Convenient, in vitro system that recapitulates many key characteristics of the adult intestinal epithelium, including intra- and intercellular signaling, self-propagating stem cell niche, and functional transport into and out of the lumen

  • Serum- and conditioned medium-free formulation

  • Suitable for use in genome editing protocols

  • Enables generation of intestinal organoids in one week

  • Complete medium formulation that delivers consistent results

Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. DMEM/F-12 with 15 mM HEPES
    DMEM/F-12 with 15 mM HEPES

    Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

  3. D-PBS (Without Ca++ and Mg++)
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

  4. Y-27632 (Dihydrochloride)
    Y-27632 (Dihydrochloride)

    RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2

Overview

IntestiCult™-SF Organoid Growth Medium (Human) is a complete cell culture medium for efficient establishment and long-term maintenance of organoids derived from human intestinal crypts. IntestiCult™-SF is a serum-free and conditioned medium-free formulation.

Intestinal organoids provide a convenient in vitro organotypic culture system for studying the intestinal epithelium. Isolated intestinal crypts rapidly form complex organoids when cultured in IntestiCult™-SF. The organoids incorporate a functional lumen enclosed by a polarized epithelial cell layer containing known cell types of the adult intestinal epithelium. Maturing intestinal organoids establish protruding buds, resembling intestinal crypts of the in vivo tissues. IntestiCult™-SF supports the proliferation of intestinal epithelial stem cells from patient’s biopsies and expansion of organoid cultures derived from fresh tissue or from already established frozen stocks.

Applications of intestinal organoid cultures include studying the development and function of intestinal epithelium, modeling intestinal diseases, and performing targeted molecule screens. Intestinal organoid cultures can also be used for investigation of adult stem cell properties and for regenerative therapy approaches, and can be further differentiated using IntestiCult™ Organoid Differentiation Medium (Human)(Catalog #100-0214).

Learn how to culture human intestinal organoids in our On-Demand Intestinal Course or browse our Frequently Asked Questions (FAQs) about the organoid workflows using IntestiCult™. Additionally, download our detailed e-book Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™ for a curated collection of intestinal organoid protocols.

Should you intend to use this product for commercial purposes, please contact HUB Organoids B.V. at www.huborganoids.nl for a commercial use license or for clarifications in relation to HUB Organoids B.V. licensing.
Subtype
Specialized Media
Cell Type
Intestinal Cells
Species
Human
Application
Organoid Culture
Brand
IntestiCult
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids, Stem Cell Biology
Formulation Category
Serum-Free

Data Figures

Organoids Grown in IntestiCult™-SF Have a Comparable Morphology to Organoids Grown in IntestiCult Organoid Growth Medium (Human)

Figure 1. Organoids Grown in IntestiCult™-SF Have a Comparable Morphology to Organoids Grown in IntestiCult Organoid Growth Medium (Human)

Human intestinal organoids grown in IntestiCult™-SF and IntestiCult™ Organoid Growth Medium (Human) display similar morphological characteristics during organoid maintenance and expansion. Shown are organoids from small intestinal and colonic tissues, established and expanded in IntestiCult™-SF or IntestiCult™ OGMH. Organoids are imaged at passage 5 of culture for all images. Scale bars = 500 μm.

IntestiCult™-SF Provides More Efficient Expansion of Intestinal Organoids Compared to IntestiCult™ OGMH

Figure 2. IntestiCult™-SF Provides More Efficient Expansion of Intestinal Organoids Compared to IntestiCult™ OGMH

Human intestinal organoids established from both (A) small intestinal and (B) colonic tissues display more efficient expansion during extended culture when grown in IntestiCult™-SF compared to those grown in IntestiCult™ OGMH. Shown is the cumulative expansion of organoids averaged across two separate donor lines in each medium. IntestiCult™-SF demonstrates more efficient expansion of both small intestinal and colonic organoids during extended culture.

Organoids Grown in IntestiCult™-SF Display Some Characteristics of the Mature Intestinal Epithelium

Figure 3. Organoids Grown in IntestiCult™-SF Display Some Characteristics of the Mature Intestinal Epithelium

While organoids grown in IntestiCult™-SF display a primarily "immature" phenotype including expression of (A) intestinal stem cell marker OLFM4 and (B) proliferation marker ki67. (C, D) These organoids also display some characteristics of the mature epithelium such as the presence of goblet cells (C, Muc2), enterocytes (C, KRT20), and enteroendocrine cells (D, CHGA). Scale bars = 50 μm or 20 μm as indicated.

Organoids Grown in IntestiCult™-SF Display Similar Genetic Expression Profiles to Those Grown in IntestiCult™ OGMH

Figure 4. Organoids Grown in IntestiCult™-SF Display Similar Genetic Expression Profiles to Those Grown in IntestiCult™ OGMH

Analysis of intestinal organoids grown in IntestiCult™-SF by qPCR of (A) Lgr5, (B) Muc2, (C) Krt20, and (D) ChgA demonstrates similar genetic expression profiles to those grown in IntestiCult™ OGMH, as well as to colonic and small intestinal tissue (A-D; x and + respectively). All expression levels are shown relative to ACTB and TBP house-keeping genes (HKGs). (E) Further analysis of three separate donors grown in IntestiCult™-SF (circles) IntestiCult™ OGMH (triangles), and published medium (squares) by principle component analysis demonstrates that differences between cultures are primarily due to donor variability with samples forming distinct clusters separated by donor, rather than by medium.

IntestiCult™-SF Enables Efficient Expansion of Intestinal Organoids from Polyp Tissue

Figure 5. IntestiCult™-SF Enables Efficient Expansion of Intestinal Organoids from Polyp Tissue

Intestinal organoids were established from both normal epithelial tissue, as well as from polyp tissue, and expanded for three passages in IntestiCult™-SF. Shown are cultures from two separate donors, imaged at the end of each passage immediately before passaging.

Organoids Grown in IntestiCult™-SF can be Further Differentiated using IntestiCult™ Organoid Differentiation Medium

Figure 6. Organoids Grown in IntestiCult™-SF can be Further Differentiated using IntestiCult™ Organoid Differentiation Medium

Further differentiation of organoids in IntestiCult™-SF can be achieved by passaging organoid cultures in IntestiCult™ Organoid Differentiation Medium as organoid-derived monolayers (2D Monolayer Diff) or in 3D organoid culture (3D Organoid Diff). Upon differentiation, markers for enterocytes (KRT20, ApoB), goblet cells (Muc2), and enteroendocrine cells (ChgA) are upregulated compared to organoids grown in IntestiCult™-SF.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
IntestiCult™-SF Organoid Growth Medium (Human)
Catalog #
100-0340
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
IntestiCult™-SF Organoid Growth Medium (Human)
Catalog #
100-0340
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
View All

Resources and Publications

Educational Materials (46)

Organoids
Brochure
Organoids
Intestinal Organoids
Brochure
Intestinal Organoids
Culturing Cancer-Derived Organoids Using IntestiCult™ Organoid Growth Medium (Human)
Technical Bulletin
Culturing Cancer-Derived Organoids Using IntestiCult™ Organoid Growth Medium (Human)
Forskolin-Induced Swelling of Human Intestinal Organoids Grown in IntestiCult™
Technical Bulletin
Forskolin-Induced Swelling of Human Intestinal Organoids Grown in IntestiCult™
Culturing Intestinal Cancer-Derived Organoids
Protocol
Culturing Intestinal Cancer-Derived Organoids
How to Perform Immunocytochemistry (ICC) Staining of Epithelial Cells Cultured as Monolayers or at the Air-Liquid Interface
Protocol
How to Perform Immunocytochemistry (ICC) Staining of Epithelial Cells Cultured as Monolayers or at t...
Barrier Permeability Assay Using Human Intestinal Organoid-Derived Monolayers
Protocol
Barrier Permeability Assay Using Human Intestinal Organoid-Derived Monolayers
How to Isolate Human Colonic Crypts from Intestinal Biopsies
Protocol
How to Isolate Human Colonic Crypts from Intestinal Biopsies
How to Cryopreserve Intestinal Organoids
Protocol
How to Cryopreserve Intestinal Organoids
CRISPR-Cas9 Genome Editing of Human Intestinal Organoids
Protocol
CRISPR-Cas9 Genome Editing of Human Intestinal Organoids
Performing Immunocytochemical Staining of Epithelial Organoids
Protocol
Performing Immunocytochemical Staining of Epithelial Organoids
How to Thaw Intestinal Organoids
Protocol
How to Thaw Intestinal Organoids
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
Protocol
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
SnapShot: GI Tract Development
Wallchart
SnapShot: GI Tract Development
SnapShot: The Intestinal Crypt
Wallchart
SnapShot: The Intestinal Crypt
SnapShot: Growing Organoids from Stem Cells
Wallchart
SnapShot: Growing Organoids from Stem Cells
Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope
1:07
Video
Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope
How to Count Intestinal Crypts for Mouse Organoid Cultures
1:09
Video
How to Count Intestinal Crypts for Mouse Organoid Cultures
Why Culture Intestinal Organoids with IntestiCult™ Organoid Growth Medium
3:19
Video
Why Culture Intestinal Organoids with IntestiCult™ Organoid Growth Medium
Collaborating to Accelerate COVID-19 Research
3:36
Video
Collaborating to Accelerate COVID-19 Research
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
24:56
Webinar
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
Patient-Derived Organoids for Drug Screening and Development
50:25
Webinar
Patient-Derived Organoids for Drug Screening and Development
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
1:10:09
Webinar
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
Nature Research Round Table: The Promise of Organoid Medicine
17:17
Webinar
Nature Research Round Table: The Promise of Organoid Medicine
Nature Research Round Table: Self-Organization and Symmetry Breaking in Intestinal Organoid Development
11:52
Webinar
Nature Research Round Table: Self-Organization and Symmetry Breaking in Intestinal Organoid Developm...
Spotlight on Organoids: Expert Panel
Webinar
Spotlight on Organoids: Expert Panel
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
17:20
Webinar
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
Nature Research Round Table: Organoid Modeling of Stem Cells and Disease Microenvironments
4:47
Webinar
Nature Research Round Table: Organoid Modeling of Stem Cells and Disease Microenvironments
Adding Hepatic Organoids to Your Research
50:59
Webinar
Adding Hepatic Organoids to Your Research
Nature Research Round Table: Organoids as Models for Human Disease
15:10
Webinar
Nature Research Round Table: Organoids as Models for Human Disease
Challenges in Translating iPSC Technology
41:47
Webinar
Challenges in Translating iPSC Technology
Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
56:31
Webinar
Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
ISSCR Innovation Showcase: Applications of Organoid and Organotypic Cultures in Infectious Diseases, Nephrotoxicity, and Highly Relevant Cell-Based Assay
59:52
Webinar
ISSCR Innovation Showcase: Applications of Organoid and Organotypic Cultures in Infectious Diseases,...
Modeling Host-Microbe Interactions Using Human Intestinal Organoids
16:31
Webinar
Modeling Host-Microbe Interactions Using Human Intestinal Organoids
Organoid Expert Panel
52:35
Webinar
Organoid Expert Panel
Nature Research Round Table: Modeling Congenital Pediatric Diarrhea in Intestinal Enteroids
15:15
Webinar
Nature Research Round Table: Modeling Congenital Pediatric Diarrhea in Intestinal Enteroids
Nature Research Round Table: Modeling Intestinal Development In Vivo and In Vitro
10:17
Webinar
Nature Research Round Table: Modeling Intestinal Development In Vivo and In Vitro
Intestinal Organoid Culture
Mini Review
Intestinal Organoid Culture
Highly Efficient Differentiation of Human Pluripotent Stem Cells into Long-term Expandable “Mini-gut” Organoids
Scientific Poster
Highly Efficient Differentiation of Human Pluripotent Stem Cells into Long-term Expandable “Mini-g...
A Serum-free Medium For Expanding Patient-derived Cystic Fibrosis Rectal Organoids And Performing Functional Assays
Scientific Poster
A Serum-free Medium For Expanding Patient-derived Cystic Fibrosis Rectal Organoids And Performing Fu...
Dr. Bas Trietsch
Interview
Dr. Bas Trietsch
Heather McCauley, PhD
Interview
Heather McCauley, PhD
Jason Spence
Interview
Jason Spence
Tamara Zietek, PhD
Interview
Tamara Zietek, PhD
Caroline Lindemans, MD, PhD
Interview
Caroline Lindemans, MD, PhD
Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™
E-Book
Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™

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Legal Statement:

This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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