How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
Protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus and will provide step by step instructions for plate coating
In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus. This video will provide step by step instructions for plate coating.
0:00 - Intro
0:29 - Matrigel Plate Coating
2:42 - Vitronectin XF™ Plate Coating
Watch the other videos in the series below:
Video 2: Generation of Cell Aggregates/Passaging
Video 3: Maintenance and Morphological Assessment
Learn more about mTeSR™ Plus hPSC maintenance media >
View a written protocol on how to transition hPSCs from feeder-free media to mTeSR™ Plus.
Publish Date:
May 20, 2020
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How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free Media
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How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
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How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
Publish Date: May 20, 2020
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STEMdiff™ Trilineage Differentiation Kit
Functional assay kit to assess pluripotency by directed differentiation of human ES and iPS cells to all three germ layers -
cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells -
qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells -
cGMP, enzyme-free human pluripotent stem cell selection and passaging reagent
