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How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus

Protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus and will provide step by step instructions for plate coating

In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus. This video will provide step by step instructions for plate coating. 0:00 - Intro 0:29 - Matrigel Plate Coating 2:42 - Vitronectin XF™ Plate Coating Watch the other videos in the series below: Video 2: Generation of Cell Aggregates/Passaging Video 3: Maintenance and Morphological Assessment Learn more about mTeSR™ Plus hPSC maintenance media > View a written protocol on how to transition hPSCs from feeder-free media to mTeSR™ Plus.
Publish Date: May 20, 2020

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