How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus. This video will provide step by step instructions for plate coating.
0:00 - Intro
0:29 - Matrigel Plate Coating
2:42 - Vitronectin XF™ Plate Coating
Watch the other videos in the series below:
Video 2: Generation of Cell Aggregates/Passaging
Video 3: Maintenance and Morphological Assessment
Learn more about mTeSR™ Plus hPSC maintenance media >
View a written protocol on how to transition hPSCs from feeder-free media to mTeSR™ Plus.
Publish Date: May 20, 2020
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