ISSCR Innovation Showcase: Advanced Brain Organoid Co-Culture Systems
In this Innovation Showcase talk from ISSCR 2020 Virtual, Dr. Erin Knock gives a detailed overview of advanced brain organoid co-culture systems.
Organoid technology is developing at a rapid pace with the field working to develop relevant disease models that are as close to human tissue as possible. In this talk she walks through some critical factors to consider before embarking on a study using organoid models. Beginning with a brief note on the importance of pluripotent stem cell (PSC) quality, she touches on how PSC-based models can inform and build upon work using animals. She then reviews some published examples of how increasingly complex PSC-derived culture systems are being used for disease modeling. Finally, she demonstrates the performance of three new PSC-based culture systems: the STEMdiff™ Brain-Region-Specific Organoid Kits for dorsal and ventral forebrain organoids, and the STEMdiff™ Microglia Culture Systems. She outlines how to use these systems and shows how to combine them as co-cultures relevant to disease modeling.
As a supporter of, and participant in, ISSCR’s 2020 Annual Meeting, we share with you our Innovation Showcase presentation at #ISSCR2020.
This presentation does not represent an endorsement from or support of the ISSCR.
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cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells -
STEMdiff™ Ventral Forebrain Organoid Differentiation Kit
Cell culture medium kit for robust generation of ventral forebrain neural organoids from human pluripotent stem cells -
STEMdiff™ Microglia Differentiation Kit
Differentiation kit for the generation of microglia precursors from human ES and iPS cell-derived hematopoietic progenitor cells -
STEMdiff™ Microglia Maturation Kit
Maturation kit for the generation of microglia from human ES and iPS cell-derived microglia precursors
