STEMdiff™ Microglia Differentiation Kit

Differentiation kit for the generation of microglia precursors from human ES and iPS cell-derived hematopoietic progenitor cells

STEMdiff™ Microglia Differentiation Kit

Differentiation kit for the generation of microglia precursors from human ES and iPS cell-derived hematopoietic progenitor cells

STEMdiff™ Microglia Differentiation Kit
1 Kit
711 USD
Catalog # 100-0019

Differentiation kit for the generation of microglia precursors from human ES and iPS cell-derived hematopoietic progenitor cells

Product Advantages


  • Greater than 90% of source HPCs are differentiated into microglia.

  • Upstream compatibility with HPCs generated using the STEMdiff™ Hematopoietic Kit and co-culture with STEMdiff™ neural organoids.

  • Allows differentiation to microglia with few contaminating macrophages or monocytes.

  • Microglia generated with the kit demonstrate phagocytosis and activation.

  • Required materials are provided in a simple, easy-to-use format.

What's Included

  • STEMdiff™ Microglia Differentiation Kit (Catalog #100-0019)
    • STEMdiff™ Microglia Basal Medium, 90 mL
    • STEMdiff™ Microglia Supplement 1, 10 mL
    • STEMdiff™ Microglia Supplement 2, 400 uL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

The STEMdiff™ Microglia Culture System comprises STEMdiff™ Microglia Differentiation Kit and STEMdiff™ Microglia Maturation Kit. Together, these kits are used to differentiate and mature microglia derived from human pluripotent stem cells (hPSCs) using STEMdiff™ Hematopoietic Kit (Catalog #05310).

Based on the protocol from the laboratory of Mathew Blurton-Jones (Abud et al., 2017), the resulting cells are a highly pure population of microglia (> 80% CD45/CD11b-positive, > 50% TREM2-positive microglia; < 20% morphologically distinct monocytes or macrophages).

Cells derived using these products are versatile tools for modeling neuroinflammation, studying human neurological development and disease, co-culture applications, and toxicity testing.
Subtype
Specialized Media
Cell Type
Hematopoietic Cells, PSC-Derived, Microglia, Neural Cells, PSC-Derived
Species
Human
Application
Cell Culture, Differentiation
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Immunology, Neuroscience
Formulation
Serum-Free

Data Figures

Experimental protocol schematic for generating microglial precursors from hPSC-derived hematopoietic progenitor cells.

Figure 1. Schematic for the STEMdiff™ Microglia Culture System Protocol

Microglial precursors can be generated in 24 days from hPSC-derived hematopoietic progenitor cells. For the generation of hematopoietic progenitor cells, see documentation for STEMdiff™ Hematopoietic Kit (Catalog #05310). For the maturation of microglial precursors to functional microglia, see the PIS.

RNA-seq profiles of hPSC-derived microglia and other immune cell types are plotted after principal components analysis.

Figure 2. Microglia Generated with STEMdiff™ Microglia Culture System Are Transcriptionally Similar to Those from Published Differentiation and Maturation Protocols

RNA-seq datasets were extracted from 4 different publications (protocols A-D) that generated hPSC-derived microglia and compared their transcriptional profiles to those of other immune cell types (N = 500 genes). Principal component analysis (PCA) was performed on these data along with RNA-seq data from microglia generated with STEMdiff™ Microglia Culture System. The hPSC-derived microglia from STEMdiff™ Microglia Culture System plot most closely to those from protocols A and B.

Time lapse of functional microglia phagocytosing bioindicator particles in culture. Over time, cells turn red and aggregate.

Figure 3. STEMdiff™ Microglia Culture System Generates Functional Microglia Capable of Phagocytosis at Day 34

Microglia taking up pH-sensitive bioindicator particles at a concentration of 250 μg/mL (small dots) were measured over a 12-hour time period with live cell imaging. As the particles are phagocytosed, the cells turn red. Over time, the number of small dots decreased, and the red cells increased in number and aggregated. Scale bar = 100μm.

Figure 4. PSC-Derived Microglia Incorporate into Brain Organoids After 10 Days and Display an Activated Morphology upon Injury.

(A) Representative microglia and brain organoid co-cultures after 10 days, stained with IBA1 for microglia (green) and MAP2 for neurons (magenta). The microglia integrate among the neurons and display an unactivated morphology with extended processes (arrow). (B) The microglia display an activated amoeboid morphology upon injury as shown by IBA1 staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0019
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0019
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0019
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0019
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (20)

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