Type I Collagen is the most abundant type of collagen in the human body and is a major structural protein prevalent in skin, bone, tendon, and other fibrous connective tissues. All collagens are characterized by a secondary protein structure consisting of three polypeptide chains wrapped around one another in a triple helical arrangement. The different types of collagen vary in the primary amino acid sequence of their polypeptide chains, with type I collagen being comprised of two identical α1(I) chains and one α2(I) chain that spontaneously form the triple helix in solution.

CellAdhere™ Type I Collagen, Human, Lyophilized is prepared from extracellular matrix secreted by normal human fibroblasts and is xeno-free. When reconstituted with 5 mL of 0.01 N HCl, the resulting concentration is 3 mg/mL.

This product is purified using a highly controlled manufacturing process with validated steps to ensure inactivation of possible viral contaminants, and it is sterility tested. The endotoxin level is 5.0 EU/mL or less, as assayed by Limulus amoebocyte lysate (LAL) assay.

This product is ideal for coating surfaces with a thin layer of protein to support cell attachment, but is not suitable for 3D cell culture applications. The optimal protein concentration may vary depending on the cell type being used, and therefore must be titrated for best results.