Human Pluripotent Stem Cell-Derived Endoderm qPCR Array

For characterizaton of hPSC-derived progenitor cells of definitive endoderm, hepatic, intestinal, or pancreatic lineages
Catalog #
07531_C
For characterizaton of hPSC-derived progenitor cells of definitive endoderm, hepatic, intestinal, or pancreatic lineages
From: 179 USD
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Required Products
  1. qPCR Master Mix Kit|07516
    qPCR Master Mix Kit

    For probe-based assays and arrays

  2. Axygen® Sealing Film for Real-Time PCR (qPCR)
    Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)

    Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)

  3. Total RNA Purification Kit
    Total RNA Purification Kit

    For purification of RNA from cells

  4.  STEMscript™ cDNA Synthesis Kits|79003
    STEMscript™ cDNA Synthesis Kits

    For reverse transcription of RNA before qPCR or RNA sequencing

Overview

The Human Pluripotent Stem Cell (hPSC)-Derived Endoderm Quantitative Polymerase Chain Reaction (qPCR) Array is designed for characterization of hPSC-derived definitive endodermal progenitor cells and their differentiated progeny including pancreatic, hepatic, and intestinal cells. This qPCR array provides the gene expression profile of hPSC-derived endodermal cells following in vitro differentiation, and can aid in development of protocols for directed differentiation towards endodermal lineages. Genes were selected based on their demonstrated expression in hPSCs, or in hPSC-derived ectodermal, mesodermal, and endodermal lineage cells (D’Amour et al.), including pancreatic (Kroon et al.), hepatic (Hay et al.), and intestinal progenitor cells (Grapin-Botton & Melton).

qPCR is used to determine changes in steady-state mRNA levels of gene expression across multiple samples, generally normalized to the relative expression of internal control genes. Gene-specific primers amplify target sequences within cDNA pools reverse-transcribed from mRNA and, as with TaqMan® technology, hybridized sequence-specific probes provide a fluorescent signal from the 5' exonuclease activity of Taq DNA polymerase. The accumulation rate of the fluorescent signal is used to quantify the sample cDNA, and thereby the amount of mRNA in the original cell lysate.

This qPCR array contains validated primers and probes for detection of 90 genes whose expression is correlated with hPSCs or hPSC-derived ectodermal, mesodermal, and endodermal cells, or pancreatic, hepatic, and intestinal progenitor cells, as well as six endogenous (housekeeping) control genes. TBP (TATA box-binding protein) qPCR Control Template is provided as a synthetic DNA positive control. Additional gene information and a qPCR analysis tool are available at www.stemcell.com/qPCRanalysis
Components
  • Human Pluripotent Stem Cell-Derived Endoderm qPCR Array (Catalog #07531)
    • hPSC-Derived Endoderm qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Pluripotent Stem Cell-Derived Endoderm qPCR Array (Catalog #07532)
    • hPSC-Derived Endoderm qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Pluripotent Stem Cell-Derived Endoderm qPCR Array (Catalog #07533)
    • hPSC-Derived Endoderm qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Pluripotent Stem Cell-Derived Endoderm qPCR Array (Catalog #07534)
    • hPSC-Derived Endoderm qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
Cell Type
Endoderm, PSC-Derived, Hepatic Cells, Intestinal Cells, Pancreatic Cells, Pluripotent Stem Cells
Species
Human
Area of Interest
Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Pluripotent Stem Cell-Derived Endoderm qPCR Array
Catalog #
07531, 07533, 07534
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Human Pluripotent Stem Cell-Derived Endoderm qPCR Array
Catalog #
07531, 07533, 07534
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Human Pluripotent Stem Cell-Derived Endoderm qPCR Array
Catalog #
07531, 07533, 07534
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Human Pluripotent Stem Cell-Derived Endoderm qPCR Array
Catalog #
07531, 07533, 07534
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Human Pluripotent Stem Cell-Derived Endoderm qPCR Array
Catalog #
07531, 07533, 07534
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Human Pluripotent Stem Cell-Derived Endoderm qPCR Array
Catalog #
07531, 07533, 07534
Lot #
All
Language
English

Educational Materials (1)

Brochure
qPCR Arrays for Cell Characterization

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Human Pluripotent Stem Cell-Derived Endoderm qPCR Array Plate Configuration

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