EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit
Immunomagnetic positive selection kit using particle release technology
EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit
Immunomagnetic positive selection kit using particle release technology
Product Advantages
- Highly purified human PSC-derived neural crest cells isolated in less than 20 minutes
- No-wash removal of EasySep™ Releasable RapidSpheres™
- Compatible with downstream differentiation protocols
What's Included
- EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Cocktail, 2 x 1.0 mL
- EasySep™ Isolation Cocktail Enhancer, 1.0 mL
- EasySep™ Release Buffer (Concentrate), 3 x 1.0 mL
- EasySep™ Releasable RapidSpheres™ 50201, 2 x 1.0 mL
Overview
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD271+ cells, which are immediately available for downstream applications.
Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Data Figures
Figure 1. Histograms of H9 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry
Starting with a single-cell suspension of H9 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).
Figure 2. Histograms of H7 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry
Starting with a single-cell suspension of H7 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).
Figure 3. Histograms of 1C cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry
Starting with a single-cell suspension of 1C cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (8)
Related Products
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
