EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit

Immunomagnetic positive selection of human CD271+ cells from human PSC-derived neural crest cell culture using particle release technology

EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit

Immunomagnetic positive selection of human CD271+ cells from human PSC-derived neural crest cell culture using particle release technology

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Immunomagnetic positive selection of human CD271+ cells from human PSC-derived neural crest cell culture using particle release technology
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Product Advantages


  • Highly purified human PSC-derived neural crest cells isolated in less than 20 minutes

  • No-wash removal of EasySep™ Releasable RapidSpheres™

  • Compatible with downstream differentiation protocols

What's Included

  • EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Cocktail, 2 x 1.0 mL
  • EasySep™ Isolation Cocktail Enhancer, 1.0 mL
  • EasySep™ Release Buffer (Concentrate), 3 x 1.0 mL
  • EasySep™ Releasable RapidSpheres™ 50201, 2 x 1.0 mL

Overview

Easily isolate highly purified and magnetic particle-free human CD271+ cells from human pluripotent stem cell (PSC)-derived neural crest cell culture samples, using immunomagnetic positive selection, with the EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are first labeled with antibody complexes recognizing CD271 and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres™ have a releasable feature. After separation using an EasySep™ magnet, bound magnetic particles are removed from the EasySep™-isolated human PSC-derived neural crest cells using a release agent, and unwanted cells are targeted for depletion. The final isolated fraction contains highly purified CD271+ cells that are immediately ready for downstream applications such as flow cytometry or cell culture. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.

Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
Subtype
Cell Isolation Kits
Cell Type
Neural Cells, PSC-Derived
Species
Human
Sample Source
Other
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Disease Modeling, Neuroscience

Data Figures

A H9 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 74.9% and 93.8% pure.

Figure 1. Histograms of H9 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H9 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

A H7 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 47.3% and 90.8% pure.

Figure 2. Histograms of H7 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H7 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

A 1C cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 18.9% and 86.7% pure.

Figure 3. Histograms of 1C cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of 1C cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
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Language
Catalog #
100-0047
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All
Language
English
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Safety Data Sheet 1
Catalog #
100-0047
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All
Language
English
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Safety Data Sheet 2
Catalog #
100-0047
Lot #
All
Language
English
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Safety Data Sheet 3
Catalog #
100-0047
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
100-0047
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.