STEMdiff™ Neural Crest Differentiation Kit
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STEMdiff™ Neural Crest Differentiation Kit -
Label for STEMdiff™ Neural Crest Differentiation Kit -
Label for STEMdiff™ Neural Crest Differentiation Kit
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Overview
• EFFICIENT - Greater than 70% purity of multipotent neural crest cells
• MULTIPOTENT - Produces cells capable of downstream differentiation, including to peripheral neurons, chondrocytes, and osteoblasts
• VERSATILE - Compatible with human ES and iPS cells maintained in mTeSR™1, mTeSR™ Plus, or TeSR™-E8™
- STEMdiff™ Neural Induction Medium, 250 mL (Catalog #05835)
- STEMdiff™ Neural Crest Differentiation Supplement, 0.5 mL
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Data and Publications
Data
Figure 1.
Neural crest cells are produced using STEMdiff™ Neural Crest Differentiation Kit after 6 days in culture. For information on continued passage of neural crest cells, contact us at techsupport@stemcell.com
Figure 2. STEMdiff™ Neural Crest Differentiation Kit Generates a Highly Pure Population of NCCs with Minimal CNS-type Progenitors
After 6 days in culture, neural crest cells (NCCs; SOX10+, red; CD271+, light blue) outnumber CNS-type progenitors (PAX6+, green). (A) Channel merge of cells fixed 2 days after being passaged on day 6. Individual immunofluorescence channels show (B) DAPI, (C) PAX6, (D) SOX10, and (E) CD271. Scale bar = 100 μm.
Figure 3. NCCs Generated with the STEMdiff™ Neural Crest Differentiation Kit Are Multipotent
NCCs (A) were cultured for 6 days and display typical morphology. (B) Culturing NCCs using established protocols generates peripheral neurons (Peripherin, green; BRN3a, red; DAPI, blue). (C) Passaging NCCs into MesenCult™-ACF Plus Medium and then into the MesenCult™-ACF Chondrogenic Differentiation Kit generates a chondrocyte pellet (Alcian Blue, Nuclear Fast Red) with deposition of cartilage around the cells. (D) Passaging NCCs into MesenCult™-ACF Plus Medium and then into the MesenCult™ Osteogenic Differentiation Kit (Human) generates an osteoblast culture with high levels of alkaline phosphatase-positive mineral deposition. Scale bar = 500 μm (A-C), 1 mm (D).