NeuroFluor™ NeuO
Membrane-permeable fluorescent probe for the detection of live neurons
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NeuroFluor™ NeuO -
Label for NeuroFluor™ NeuO
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Overview
NeuroFluor™ NeuO is a membrane-permeable fluorescent probe that selectively labels primary and pluripotent stem cell-derived neurons in live cultures (J C Er et al. Angew Chem Int Ed, 2015). Cells labeled with NeuroFluor™ NeuO can be visualized using fluorescent imaging. Labeling with this probe is non-permanent; it can be washed off, providing unlabeled, viable cells for downstream applications. For additional information, see References.
Advantages:
• Enables selective labeling of mouse, rat, or human neurons without fixation
• Can be used to confirm neuronal differentiation of human pluripotent stem cell-derived neural progenitor cells
• Can be used to label neurons in live culture
• Non-toxic and non-permanent
• Simple and rapid labeling protocol
• Can be used to confirm neuronal differentiation of human pluripotent stem cell-derived neural progenitor cells
• Can be used to label neurons in live culture
• Non-toxic and non-permanent
• Simple and rapid labeling protocol
Contains:
• 100 µM NeuO (CAS Number: 1616355-50-0, 1668597-38-3)
• DMSO
• DMSO
Application:
Characterization; Phenotyping
Brand:
NeuroFluor
Area of Interest:
Stem Cell Biology; Neuroscience; Disease Modeling
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Scientific Resources
Product Documentation
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data and Publications
Data
Figure 1. NeuroFluor™ NeuO Selectively Labels Primary E18 Rat Neurons
(A) Primary rat E18 cortical neurons were labeled with 0.25μM NeuroFluor™ NeuO (green) and incubated for 1 hour. Image was taken after 2 hours of incubation. (B) The same culture was later fixed and stained for β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Nuclei are counterstained with DAPI. Images were taken at 20X magnification.
Figure 2. NeuroFluor™ NeuO Selectively Labels hPSC-Derived Neurons
(A) The neuronal precursors generated from hPSC-derived (XCL-1) neural progenitor cells were cultured in STEMdiff™ Neuron Maturation Medium. After 18 days of culture, hPSC-derived neurons were labeled with NeuroFluor™ NeuO (green). Nuclei are counterstained with DAPI. (B) The same culture was later fixed and stained with β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Images were taken at 20X magnification.
Publications
(1)
Angewandte Chemie - International Edition 2015 FEB
Neuo: A fluorescent chemical probe for live neuron labeling
Abstract
Abstract
To address existing limitations in live neuron imaging, we have developed NeuO, a novel cell-permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.