Figure 1. NeuroFluor™ NeuO Selectively Labels Primary E18 Rat Neurons

(A) Primary rat E18 cortical neurons were labeled with 0.25μM NeuroFluor™ NeuO (green) and incubated for 1 hour. Image was taken after 2 hours of incubation. (B) The same culture was later fixed and stained for β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Nuclei are counterstained with DAPI. Images were taken at 20X magnification.

Figure 2. NeuroFluor™ NeuO Selectively Labels hPSC-Derived Neurons

(A) The neuronal precursors generated from hPSC-derived (XCL-1) neural progenitor cells were cultured in STEMdiff™ Neuron Maturation Medium. After 18 days of culture, hPSC-derived neurons were labeled with NeuroFluor™ NeuO (green). Nuclei are counterstained with DAPI. (B) The same culture was later fixed and stained with β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Images were taken at 20X magnification.