Human Recombinant bFGF, ACF

Basic fibroblast growth factor, animal component-free
Catalog #
78134_C
Basic fibroblast growth factor, animal component-free
From: 104 USD

Overview

Basic fibroblast growth factor (bFGF) is a prototypic member of the fibroblast growth factor family. Cytokines in the FGF family possess broad mitogenic and cell survival activities (Folkman & Klagsbrun; Kimelman & Kirschner) and are involved in a variety of biological processes including cell proliferation, differentiation, survival, and apoptosis (Folkman & Klagsbrun; Klagsbrun; Rifkin & Moscatelli). bFGF has the β-trefoil structure (Ponting & Russell), binds to the four FGF receptor (FGFR) family members, and activates JAK/STAT, PI3K, ERK1/2, and other receptor tyrosine kinase (RTK) signaling pathways. It supports the maintenance of undifferentiated human pluripotent stem cells (Xu et al.; Kang et al.), stimulates human pluripotent stem cells to form neural rosettes (Zhang et al.), and improves proliferation of human mesenchymal stem cells and enhances chondrogenic differentiation (Solchaga et al.). This version of bFGF is the full-length bFGF protein encoded by the human FGF2 gene consisting of 154 amino acid residues. This product is animal component-free.
Subtype
Cytokines, Growth Factors
Alternative Names
Basic fibroblast growth factor, FGF-β, FGF2, Fibroblast growth factor-basic, HBGF-2
Cell Type
Brain Tumor Stem Cells, Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Mesenchymal Stem and Progenitor Cells, Mesoderm, PSC-Derived, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons, Pluripotent Stem Cells, Prostate Cells
Area of Interest
Epithelial Cell Biology, Neuroscience, Stem Cell Biology
Molecular Weight
17.3 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant bFGF, ACF
Catalog #
78134, 78134.1, 78134.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant bFGF, ACF
Catalog #
78134, 78134.1, 78134.2
Lot #
All
Language
English

Educational Materials (8)

Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Small Molecules for Stem Cell Research
Brochure
Products for Human Pluripotent Stem Cells
Brochure
BrainPhys™ Neuronal Medium for Improved Neuronal Function
Technical Bulletin
Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSR™1 or TeSR™-E8™
Wallchart
Stem Cell States: Naive to Primed Pluripotency
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Webinar
Methods and Culture Reagents to Maintain a Spectrum of hPSC States from Naive-Like to Primed
23:35
Methods and Culture Reagents to Maintain a Spectrum of hPSC States from Naive-Like to Primed

Product Applications

Data and Publications

Data

(A) The biological activity of Human Recombinant bFGF, ACF was tested by its ability to promote the proliferation of NR6R-3T3 cells. Cell proliferation was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.0507 ng/mL. (B) 1 μg of Human Recombinant bFGF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

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