Hematopoietic Stem and Progenitor Cell Research
Hematopoietic stem and progenitor cells (HSPCs) can be identified both phenotypically, e.g., by flow cytometry, and functionally, with colony-forming unit (CFU) or long-term culture-initiating cell (LTC-IC) assays.
Products
Cell Culture Media and Supplements (4 31)
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MethoCult™ SF H4636 Without EPO
Serum-free methylcellulose-based medium with recombinant cytokines for human hematopoietic cells -
MegaCult™-C Medium with Lipids
Medium with lipids, without cytokines, for human and mouse CFU-Mk assays -
MegaCult™-C Collagen and Medium with Lipids
Kit containing collagen and medium with lipids, without cytokines, for human and mouse CFU-Mk assays -
MegaCult™-C Complete Kit with Cytokines
Complete kit for human CFU-Mk assays
Instruments and Software (4 2)
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STEMvision™
Automated and standardized colony counting for the hematopoietic colony-forming unit (CFU) assay -
L-Calc™ Software
Software for limiting dilution analysis
Cytokines and Proteins (4 2)
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Human Recombinant FGF-acidic, ACF
Fibroblast growth factor-acidic, animal component-free -
Human Recombinant FGF-acidic
Fibroblast growth factor-acidic
Antibodies (4 13)
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Anti-Human CD45 Antibody, Clone 2D1
Mouse monoclonal IgG1 antibody against human CD45 -
Anti-Human CD3 Antibody, Clone UCHT1
Mouse (BALB/c) monoclonal IgG1 antibody against human, chimpanzee CD3 -
Anti-Mouse CD11b Antibody, Clone M1/70
Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b -
Anti-Mouse CD45R (B220) Antibody, Clone RA3-6B2
Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220)
Cell Dyes and Detection Assay Kits (4 13)
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GloCell™ Fixable Viability Dye Violet 540
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye Violet 510
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye Violet 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye UV 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
Cultureware and General Supplies (4 11)
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245 mm Square Dish, Tissue Culture-Treated
Sterile clear polystyrene tissue culture-treated dish with lid -
SmartDish™
Meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays -
Double Chamber Slide Kit
For the culture, fixation, and staining of collagen-based cultures -
STEMgrid™-6
Detachable grid for manual counting of hematopoietic colonies in SmartDish™ 6-well plates using an inverted microscope
Buffers and Solutions (4 5)
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Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum
Cell culture buffer -
ALDEFLUOR™ Assay Buffer
Assay buffer for labeling with the ALDEFLUOR™ kit -
HBSS with 10 mM HEPES, Without Phenol Red
Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red -
HBSS, Modified (Without Ca++ and Mg++)
Hanks' Balanced Salt Solution (HBSS) without calcium and magnesium
Small Molecules (4 1)
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Hydrocortisone
Enhances proliferation and differentiation of endothelial, epithelial, mesenchymal, and oligodendrocyte cells
Matrices and Substrates (4 1)
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Collagen Solution
For preparation of collagen gels and for coating cell culture surfaces
ELISAs (4 1)
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Erythropoietin (EPO) ELISA Kit
Immunoassay for detection and measurement of human erythropoietin