GloCell™ Fixable Viability Dye Violet 540
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 540 -
Label for GloCell™ Fixable Viability Dye Violet 540
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Required Products
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Cell separation buffer -
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
GloCell™ Fixable Viability Dye Violet 540 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.
GloCell™ Fixable Viability Dye Violet 540 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 540 nm that can be detected using the 525/50 band pass filter used to detect AmCyan.
GloCell™ Fixable Viability Dye Violet 540 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 540 nm that can be detected using the 525/50 band pass filter used to detect AmCyan.
Application:
Flow Cytometry; Immunofluorescence
Brand:
GloCell
Area of Interest:
Angiogenic Cell Research; Antibody Development; Cancer Research; Cell Line Development; Chimerism; Disease Modeling; Drug Discovery and Toxicity Testing; Endothelial Cell Biology; Epithelial Cell Biology; HIV; HLA; Hybridoma Generation; Immunology; Infectious Diseases; Metabolism; Mitosis; Neuroscience; Organoids; Respiratory Research; Stem Cell Biology; Cell Therapy; Transplantation Research
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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Figure 1.
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Violet 540. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Violet 540.
Figure 2.
Excitation/Emission spectra for GloCell™ Fixable Viability Dye Violet 540. Excitation max: 399 nm, Emission Max: 540 nm
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.